a. To determine: The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein). Introduction: The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

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Answer 1

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

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In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true: I. Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype II. Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type) III. Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype IV. Injection of the white experimental transgene in the rosy mutant…

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Answer 2

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 3

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 4

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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See solution

Answer 5

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 6

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Answer 7

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Answer 8

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Answer 9

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Answer 10

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Answer 11

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Answer 12

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 13

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...

Solution Summary: The author describes gene trapping as a high-throughput approach that is utilized for the insertional type of mutations across the genome of mammals.

Concept explainers

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b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Want to see the full answer?

Chapter 18 Solutions

Solution Summary: The author describes gene trapping as a high-throughput approach that is utilized for the insertional type of mutations across the genome of mammals.

Concept explainers

Videos

b. To determine: Whether the genome of all mice should be integrated in a way which is described in the previous part. Introduction: A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

c. To determine: The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques. Introduction: The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Want to see the full answer?

Chapter 18 Solutions

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.