Concept explainers
a.
To determine:
The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).
Introduction:
The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.
b.
To determine:
Whether the genome of all mice should be integrated in a way which is described in the previous part.
Introduction:
A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.
c.
To determine:
The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.
Introduction:
The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.
d.
To determine:
The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.
Introduction:
The constructs of DNA (Deoxyribose
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GENETICS:FROM GENES TO GENOMES-ACCESS
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- The MAT locus allows yeast to switch mating type through a very complex mechanism. However, it has informed us a great deal about what aspects of gene expression typical to all organisms? Options: higher order changes in chromatin affect transcriptional efficiency that general transcription factors must first bind directly to histone tails and only then can they interact with their cognate binding sites that DNA methylation is involved in this silencing mechanism that SIR2 is required for all types of transcriptional repression that expression of Pol III genes provides a means of identifying active chromatinarrow_forwardGlenn Croston and his colleagues studied the relation between chromatin structure and transcription activity. In one set of experiments, they measured the level of in vitro transcription of a Drosophila gene by RNA polymerase II in the presence of DNA and various combinations of histone proteins (G. E. Croston et al. 1991. Science 251:643–649). First, they measured the level of transcription of naked DNA, with no associated histone proteins. Then they measured the level of transcription after nucleosome octamers (without H1) were added to the DNA. The addition of the octamers caused the level of transcription to drop by 50%. When both nucleosome octamers and H1 proteins were added to the DNA, transcription was greatly repressed, dropping to less than 1% of that obtained with naked DNA, as shown in the table below. GAL4-VP16 is a protein that binds to the DNA of certain eukaryotic genes. When GAL4-VP16 is added to DNA, the level of transcription by RNA polymerase II is greatly elevated.…arrow_forward. Let’s say that you have incredible skill and can isolate the white and red patches of tissue from the Drosophila eyes shown in Figure 12-24 in order to isolate mRNA from each tissue preparation. Using your knowledge of DNA techniques from Chapter 10, design an experiment that would allow you to determine whether RNA is transcribed from the white gene in the red tissue or the whitetissue or both. If you need it, you have access to radioactive white-gene DNAarrow_forward
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