Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Textbook Question
Chapter 18.5, Problem 1MQ
- What could you conclude from PCR/DGGE analysis of a sample that yielded one band by PCR and one band by DGGE? One band by PCR and four bands by DGGE?
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how to find approximate sizes of each band and their relative brightness in pcr?
If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?]
What is the final volume of the individual PCR reactions we are making?
If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?
Chapter 18 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 18.1 - Describe the enrichment strategy behind...Ch. 18.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 18.1 - What is enrichment bias? How does dilution reduce...Ch. 18.2 - How does the agar dilution method differ from...Ch. 18.2 - How might you isolate a morphologically unique...Ch. 18.2 - What is meant by high-throughput in culturing...Ch. 18.3 - How does viability staining differ from stains...Ch. 18.3 - Prob. 2MQCh. 18.3 - Why is it incorrect to say that the GFP is a...Ch. 18.4 - What structure in the cell is the target for...
Ch. 18.4 - FISH and CARD-FISH can be used to reveal different...Ch. 18.5 - What could you conclude from PCR/DGGE analysis of...Ch. 18.5 - What surprising finding has come out of many...Ch. 18.6 - MINIQUIZ
• What is a phylochip and what can it...Ch. 18.6 - What are the advantages and disadvantages of...Ch. 18.6 - Prob. 3MQCh. 18.7 - Prob. 1MQCh. 18.7 - How do environmental genomic approaches differ...Ch. 18.7 - Prob. 3MQCh. 18.8 - Prob. 1MQCh. 18.8 - If a large pulse of organic matter entered the...Ch. 18.9 - Prob. 1MQCh. 18.9 - What is the simplest explanation for why lunar...Ch. 18.9 - What is the expected isotopic composition of...Ch. 18.10 - How could NanoSIMS be used to identify a...Ch. 18.10 - Prob. 2MQCh. 18.10 - How does MAR-FISH link microbial diversity and...Ch. 18.11 - How can stable isotope probing reveal the identity...Ch. 18.11 - What key method is required to do genomics on a...Ch. 18.11 - Prob. 3MQCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQCh. 18 - Prob. 6RQCh. 18 - Prob. 7RQCh. 18 - Prob. 8RQCh. 18 - Prob. 9RQCh. 18 - REVIEW QUESTIONS
10. Why is a microarray not...Ch. 18 - Prob. 11RQCh. 18 - Prob. 12RQCh. 18 - Q What are the major advantages of radioisotopic...Ch. 18 - Prob. 14RQCh. 18 - Prob. 15RQCh. 18 - REVIEW QUESTIONS
16. What is the advantage of...Ch. 18 - Prob. 17RQCh. 18 - Design an experiment for measuring the activity of...Ch. 18 - You wish to know whether Archaea exist in a lake...Ch. 18 - Design an experiment to solve the following...Ch. 18 - Design a SIP experiment that would allow you to...
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- What would be the final primer concentration if 0.5 μl of 10 μM primers were added to a PCR reaction with a final volume of 20 μl?arrow_forwardIs the given statement that the first step of PCR process involves heating of DNA solution TRUE or FALSE?arrow_forwardHow does Sanger sequencing differ from PCR?arrow_forward
- What determines the size (length) of the primary PCR product? What might a successful gel check of a PCR reaction look like?arrow_forwardWhat are the three steps of PCR?arrow_forwardYou have a 100 micromolar stock solution of dNTPs in the freezer and are setting up a PCR reaction in which you need to have a final concentration of 20 micromolar dNTPs. In a reaction volume of 25 microliters, how much of your stock dNTPs do you add to each PCR reaction?arrow_forward
- In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwardWhat is PCR bias? Why is it undesirable?arrow_forwardYou have added 4 μl of a 5 μM stock primer to a 25 μl PCR reaction. What is the amountof primer in nanomol that you have added to the reaction?arrow_forward
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