PRESCOTT'S MICROBIOLOGY
PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 19, Problem 1RC
Summary Introduction

To determine: The structure of DNA and the methods used for DNA sequencing and metagenomics

Introduction. All the enzymes in the body that are crucial for the biochemical reactions are proteins. The proteins are made of amino acids. The amino acids are of 20 types that combine in a varied manner to form proteins. The amino acids join together by peptide bonds. Proteins act as major substrates and reactants for the metabolic pathways.

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Explanation of Solution

The DNA (deoxyribose nucleic acid) is made up of stacks of nucleotides that are joined to each other by phosphodiester bond vertically and horizontally by H-bonds. The adenine and thymine form two H-bonds while the bases guanine and cytosine form three H-bonds. The DNA is a double helical structure that has the phosphate-sugar backbone and the nitrogenous bases form the stacks of the DNA that are present towards the inner direction of the helical molecule.

The methods that are used for genomic sequencing are Sanger’s sequencing, chain termination sequencing, high-throughput sequencing methods, next-generation sequencing, and so on. The metagenomic analysis can be done with the help of bioinformatics tools like KEGG (Kyoto encyclopedia for gene and genomes) where the function and metabolic information on different genes and proteins can be identified from a large repository of data.

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Chapter 19 Solutions

PRESCOTT'S MICROBIOLOGY

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