BROCK BIO.OF MICROORGANISMS-W/ACCESS
15th Edition
ISBN: 9781323776346
Author: MADIGAN
Publisher: PEARSON C
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Textbook Question
Chapter 19.6, Problem 1MQ
- What could you conclude from PCR/DGGE analysis of a sample that yielded one band by PCR and one band by DGGE? One band by PCR and four bands by DGGE?
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how to find approximate sizes of each band and their relative brightness in pcr?
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If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?
Chapter 19 Solutions
BROCK BIO.OF MICROORGANISMS-W/ACCESS
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- What would be the final primer concentration if 0.5 μl of 10 μM primers were added to a PCR reaction with a final volume of 20 μl?arrow_forwardIs the given statement that the first step of PCR process involves heating of DNA solution TRUE or FALSE?arrow_forwardHow does Sanger sequencing differ from PCR?arrow_forward
- What determines the size (length) of the primary PCR product? What might a successful gel check of a PCR reaction look like?arrow_forwardWhat are the three steps of PCR?arrow_forwardYou have a 100 micromolar stock solution of dNTPs in the freezer and are setting up a PCR reaction in which you need to have a final concentration of 20 micromolar dNTPs. In a reaction volume of 25 microliters, how much of your stock dNTPs do you add to each PCR reaction?arrow_forward
- In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwardWhat is PCR bias? Why is it undesirable?arrow_forwardYou have added 4 μl of a 5 μM stock primer to a 25 μl PCR reaction. What is the amountof primer in nanomol that you have added to the reaction?arrow_forward
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