Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN: 9781305544703
Author: STARR
Publisher: Cengage
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Chapter 20, Problem 12SQ
Summary Introduction
Introduction: Plasmids are the extra-chromosomal circular DNA found in many bacterial cells. Plasmids are used for the bacterial conjugation. It consists of genes like antibiotic resistance gene that provides the bacterial cells with additional features. Plasmids are independent of the chromosomal DNA for their replication. Plasmids are varied in size (ranges from hundreds to thousands of DNA base pairs per plasmids).
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A plasmid is a circle of________ . a. RNA c. either RNA or DNA b. DNA d histone proteins
Which of the following characteristics is not true of a plasmid?a. It is a circular piece of DNA.b. It is required for normal cell function.c. It is found in bacteria.d. It can be transferred from cell to cell.
For A. Answer is Culture in ampicillin media to isolate bacteria that took up the plasmid, killing off the bacteria that did not take up the plasmid. Can you explain why?
For B. Answer is Lane C. Can you explain why?
Chapter 20 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 20 - Prob. 1SQCh. 20 - Prob. 2SQCh. 20 - Prob. 3SQCh. 20 - Prob. 4SQCh. 20 - __________ do not reproduce sexually. a....Ch. 20 - Prob. 6SQCh. 20 - Adapting to Host Defenses Surface proteins called...Ch. 20 - Prob. 2DAACh. 20 - Prob. 3DAACh. 20 - Adapting to Host Defenses Surface proteins called...
Ch. 20 - Prob. 7SQCh. 20 - Bacteria that serve as decomposers are ___ . a....Ch. 20 - Prob. 9SQCh. 20 - Formation of a(n) ___ allows some soil bacteria to...Ch. 20 - Prob. 11SQCh. 20 - Prob. 12SQCh. 20 - Which of these diseases is caused by bacteria? a....Ch. 20 - Prob. 14SQCh. 20 - Prob. 15SQCh. 20 - If a cut or scrape becomes infected,...Ch. 20 - The adenoviruses that cause colds do not have a...Ch. 20 - Prob. 3CTCh. 20 - Prob. 4CT
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- Plasmids are circular extrachromosomal DNA in bacteria that play a role in: d. degradation of viruses a. growth of bacteria b. pathogenicity and antibiotic resistance c. production of antibodiesarrow_forwardThe phrase 5to3 refers to the _________ . a. timing of DNA replication b. directionality of DNA synthesis c. number of phosphate groupsarrow_forwardWhy must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forward
- Which of the following statements is false? a. Plasmids can be exchanged between different species of bacteria. b. The blue circle represents a small chromosome. c. The blue circle is a plasmid that contains genes that confer resistance to specific antiobiotics. d. The blue circle represents a double-stranded molecule of DNA.arrow_forwardUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. There are NO sites where multiple enzymes cut. How many base pairs long is the plasmid? 3kb 2kb 4kb 5kbarrow_forwardA small nonessential circular DNA molecule in prokaryotes is called a: a. plasmid b. telomere c. bacteriophage d. prophagearrow_forward
- Which of the following statements is true of plasmids? a. They are composed of RNA. b. They normally exist outside of bacterial cells. c. They possess only a single strand of DNA. d. They contain an origin of replicationarrow_forwardWhich gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease b. virus receptors c. a gene for antibiotic resistance d. reverse transcriptasearrow_forwardSmall circular molecules of DNA in bacteria ____. a. result from the activity of restriction enzymes b. are plasmids c. cannot survive outside of the cell d. are eventually degraded e. are DNA fragments from their main chromosomearrow_forward
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. F the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kbarrow_forwardA segment of DNA that reads from the same forward and backward is calleda) Palindromic DNAb) Complementary DNAc) Plasmid DNAd) Copy DNAarrow_forwardChoose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forward
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