The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

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Answer 1

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Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.

In a typical PCR reaction what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.

You have a 100 micromolar stock solution of dNTPs in the freezer and are setting up a PCR reaction in which you need to have a final concentration of 20 micromolar dNTPs. In a reaction volume of 25 microliters, how much of your stock dNTPs do you add to each PCR reaction?

Answer 2

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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Answer 3

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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Answer 4

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Expert Solution & Answer

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Answer 5

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 6

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Answer 7

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Answer 8

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

Answer 9

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

Answer 10

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 11

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 12

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Answer 15

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The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Solution Summary: The author explains the polymerase chain reaction, which produces multiple copies of a specific DNA segment.

Videos

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Want to see the full answer?

Chapter 20 Solutions

The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Solution Summary: The author explains the polymerase chain reaction, which produces multiple copies of a specific DNA segment.

Videos

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Want to see the full answer?

Chapter 20 Solutions

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.