Human Biology Concepts and Current Issues; Modified MasteringBiology with Pearson EText -- ValuePack Access Card -- for Human Biology: Concepts and Current Issues
8th Edition
ISBN: 9780134560779
Author: Johnson, Michael D.
Publisher: Pearson Education
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Textbook Question
Chapter 20, Problem 3TY
Which of the following is used to separate fragments of DNA by size?
a. gel electrophoresis
b. polymerase chain reaction
c. DNA insertion into a plasmid
d. DNA splicing
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From where do we get primers for sequencing DNA?
A) they are synthesized by reverse transcriptase
B) they are cut out of plasmids using restriction endonucleases
C) DNA primase is added to the sequencing reaction and synthesizes the primers
D) biotechnology companies synthesize them using organic chemistry
Choose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmid
Explain how electrophoresis separates DNA strands.
a. How is a DNA fingerprinting test interpreted?
b. Define plasmid and how plasmids can change a bacteria’s activity.
c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme.
d. Define sticky end and blunt end and which one is useful in molecular biology.
Chapter 20 Solutions
Human Biology Concepts and Current Issues; Modified MasteringBiology with Pearson EText -- ValuePack Access Card -- for Human Biology: Concepts and Current Issues
Ch. 20 - Prob. 1QCCh. 20 - GM foods are created for a variety of reasons,...Ch. 20 - Should foods that contain genetically modified...Ch. 20 - Explain how DNA is sequenced.Ch. 20 -
2. Describe the enzymes used in recombinant DNA...Ch. 20 - Prob. 3CRCh. 20 - Discuss the polymerase chain reaction technique,...Ch. 20 - Prob. 5CRCh. 20 - Prob. 6CRCh. 20 - Prob. 7CR
Ch. 20 - Prob. 8CRCh. 20 - Prob. 9CRCh. 20 -
10. List the diseases that gene therapy may be...Ch. 20 - Prob. 1TYCh. 20 - Prob. 2TYCh. 20 - Which of the following is used to separate...Ch. 20 - Genetically engineered human insulin, human growth...Ch. 20 - Prob. 5TYCh. 20 - All of the following statements about transgenic...Ch. 20 -
7. "Gene pharming” refers to:
a. using transgenic...Ch. 20 - Prob. 8TYCh. 20 - Prob. 9TYCh. 20 - Prob. 10TYCh. 20 - Which of the following is needed for sequencing...Ch. 20 - A tiny amount of blood was recovered from a crime...Ch. 20 - Which technique will allow amplification of the...Ch. 20 - A gene for a bacterial protein with insecticide...Ch. 20 - Prob. 15TYCh. 20 -
1. What do you think are the chances that someone...Ch. 20 - Imagine a future in which the respiratory effects...Ch. 20 - Prob. 3AWKCh. 20 - Prob. 4AWKCh. 20 - Prob. 5AWKCh. 20 - There is interest in producing vaccines in foods....Ch. 20 - Transgenic plants have the potential to improve...
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- Why is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple copies of itself. b.) It joins two DNA fragments together. c.) It shapes bacterial DNA into a circular plasmid. d.) It cuts DNA into restriction fragments.arrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardWhy must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forward
- Which type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertionarrow_forwardPCR is essential for Select one: A. allowing restriction enzymes to cut DNA. B. making many copies of a small amount of DNA. C. cutting DNA into many small pieces. D. creating recombinant plasmids.arrow_forwardThe polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNAarrow_forward
- Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardWhich of the following statements applies to both point and frameshift mutations? A. A base is removed from a DNA template strand B. A base is added to a DNA template strand C. A base is substituted for another base in a DNA template strand D. No correct responsearrow_forwardVariable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in: A) Recombinant DNA technology B) Monoclonal antibody production C) Stemcell culture D) DNA fingerprintingarrow_forward
- A protein that can cut DNA at specific DNA base sequences is called aa. DNase. c. restriction enzyme.b. DNA ligase. d. DNA polymerase.arrow_forwardA DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forwardWhat do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together? a.) an amino acid sequence b.) DNA ligase c.) restriction enzymes d.) mRNAarrow_forward
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