Biology
4th Edition
ISBN: 9781259188121
Author: Peter Stiling, Robert Brooker, Linda Graham, Eric Widmaier
Publisher: McGraw-Hill Education
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Chapter 20.1, Problem 2CC
Summary Introduction
To determine: The purpose of the lacZ gene in the vector in the cloning experiment.
Introduction: Genomics contains an important technique by which multiple and exact copies of a gene can be produced. This technique is known as gene cloning. This method requires certain agents such as plasmids and viruses for the successful production of multiple genes from a specific gene. These are known as vectors. Vectors carry the gene of interest in the host cell and thus, help in gene cloning.
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Chapter 20 Solutions
Biology
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 1BCCh. 20.1 - Prob. 2CCCh. 20.1 - Prob. 3CCCh. 20.1 - Prob. 4CCCh. 20.1 - Prob. 2BCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.2 - Prob. 3CCCh. 20.3 - Prob. 1CC
Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.3 - Prob. 4CCCh. 20.3 - Prob. 1EQCh. 20.3 - Prob. 2EQCh. 20.3 - Prob. 3EQCh. 20 - Prob. 1TYCh. 20 - Prob. 2TYCh. 20 - DNA ligase is needed in a cloning experiment a. to...Ch. 20 - Prob. 4TYCh. 20 - Why is Taq polymerase used in PCR rather than...Ch. 20 - Lets suppose you want to clone a gene that has...Ch. 20 - Prob. 7TYCh. 20 - Prob. 8TYCh. 20 - Prob. 9TYCh. 20 - Prob. 10TYCh. 20 - Prob. 1CQCh. 20 - Prob. 2CQCh. 20 - Prob. 3CQCh. 20 - Identify and discuss three important advances that...Ch. 20 - Prob. 2COQ
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- Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.arrow_forwardHi, this is my Recombinant "Paper" Plasmid activity question, I did other parts, but this question I really have no clue what it is. Question: What polypeptide sequence does the inserted gene code for? (Review protein synthesis and write the full amino acid sequence for the inserted gene.) in the picture attached: I Draw a ring symbolizing my plasmid that has been recombined with another gene. it shows the following: • where inserted gene is located on your loop. • where the origin of replication is located. • which antibiotic resistant genes (R – genes) I preserved and what sequential order is • Name and where your restriction enzymes (endonucleases) cut.arrow_forwardPreforming a "blue-white screen" 3) Would bacteria that have taken up a plasmid into which a DNA fragment has been inserted, form a blue colony or a white colony when grown on this medium? Briefly explain why these bacteria would form a colony of the color you chose.arrow_forward
- gene. If the JM109 strain is transformed by the PBKSK plasmid, the strain will produce the B-galactosidase (from the lac gene) and will hydrolyze X-gal to produce the blue compound. Therefore, colonies that were transformed and contain the pBSKS wil you appear blue. IPTG & X-Gal & NO colonies Amp E. coli JM109 E. coli JM109 50 mM calcium chloride-15% glycerol lac lac lac IPTG & I Recovery X-Gal solution at -702C PBSKS White colonies E. coli JM109 E. coli JM109 ampR amp I amp lac lac Heat Shock Non-transformed 42°C E. coli JM109 E. coli JM109 amps amps lac lac IPTG & X-Gal lac I Recovery lac PBSKS BLUE colonies PBSKS ampRI (amp Transformed IPTG & X-Gal & BLUE colonies Amp Hypotheses: Circle the correct answer 1. If PBSKS is transformed into JM109 cells, colonies will be (able/not able) to grow in the presence of ampicillin. a. Why? _ 2. If PBSKS is transformed into JM109 cells, colonies in media with IPTG (will/will not) induce the production the B- galactosidase enzyme. a. Why?_ 3. If…arrow_forward19. Which of the following statements is true for tumor inducing plasmids? Group of answer choices This plasmid can induce tumors in all plants The tumor kills the host organism soon after induction Their use provides an efficient method for expressing a eukaryotic gene in a prokaryote This plasmid comes from a eukaryotic organism The plasmid has a region used to produce opines where new genetic information can be addedarrow_forward. A second feature that virtually all plasmid vectors havein common is the selectable marker. Explain what this isand why it is such an important feature.arrow_forward
- Antisense RNA If MRNA is produced using this plasmid, what changes would result in production of an antisense RNA? Gene A Promoter McGraw-Hill Educston Check All That Apply No change is necessary because the MRNA Is the antisense RNA. The promoter must be moved to the other slde of Gene A. The antisense DNA must be Inserted Into the plasmid. Reverse the direction of Gene A In the plasmid.arrow_forwardAnimal Cell DNA Bacterial cell Plasmid Bacterial 1 3 5 chromosome Questions: 1. Why are plasmids used as vector for DNA Recombination? What other vectors can be used? 2. How are Recombinant DNA formed? 3. What is the difference between genetic modification and selective breeding? EXCELLEarrow_forwardDefine/Compare/Contrast/Distinguish these three terms: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)arrow_forward
- 1/ Why is Thermus aquaticus so useful: O t is necessary for the mass production of bacteria containing a plasmid with an inserted gene O It is used in automated DNA sequencing O It facilitates the polymerase chain reaction O It is used to create recombinant plasmidsarrow_forwardPriming for virus DNA replication can happen by all of the following EXCEPT: O a. Primase adding a RNA primer O b.Recognition of a promoter region Oc. Self-priming by formation of a hairpin secondary structure at the ends which have inverted terminal repeats (ITRS) O d.Using a protein (example: terminal protein at the 5' end of the genome) 1 F If there was no reverse transcriptase available, a DNA virus could still replicate its genome. O True O False Seve All Answersarrow_forwardRestriction mapping of a plasmid: Digestion of a plasmid pCHEM1234 with the following restriction enzymes (single and double-cut) resulted in the fragments below. Draw a restriction map of the plasmid using the data provided below A. PCHEM 1234. Shown below are the restriction fragments BamHI 52 kb Hind III 26, 12, 8, 6 kb BamHI and HindIII double digest 14, 12, 8, 6 kbarrow_forward
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