Concept explainers
To explain: The role of complementary base-pairing in RT-PCR, DNA microarray analysis, RNA sequencing, and CRISPR-Cas9 editing.
Concept introduction:
The gene functions can be studied through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to the gene of interest are to be identified. The methods used for these include RT-PCR, DNA microarray analysis, and RNA sequencing. These are based on the base-pairing between the
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Bio 121 Campbell Biology Truman College
- Briefly and in simple terms, describe how CRISPR could be utilized in treating genetic conditions such as Batten disease.arrow_forwardDirect modification of genes may be achieved using what? (Choose one below): DNA microarray CRISPR-Cas system Hybridizationarrow_forwardConsider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain cancer. Which are the best candidates for treatment with CRISPR-Cas genome editing, and which have the largest hurdles to overcome? Why?arrow_forward
- Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.arrow_forwardPlease place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect and state why? Sequence Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Clonal isolation Synthesise DNA insert oligonucleotides Clonal characterisation (analysis of phenotype) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Transfect cells Clone into CRISPR-cas9 expression vector Purify plasmids Design a set of targeting sequences using algorithms.arrow_forwardExplain How Single-nucleotide mutations can be introduced into the genome using an engineered CRISPR-Cas9 system.arrow_forward
- Microarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.arrow_forwardDescribe the features of a typical CRISPR locus in a bacterial cell.arrow_forwardWhat is the function of the CRISPR/Cas system? What are the ethical impacts of a technology such as CRISPR?arrow_forward
- What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.arrow_forwardDescribe how the genome editing tool CRISPR-Cas9 works.arrow_forwardwhat are the advantages and disadvantages of CRISPR cas9 technology?arrow_forward
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