PRESCOTT'S MICROBIO W/PROCTORIO
PRESCOTT'S MICROBIO W/PROCTORIO
11th Edition
ISBN: 9781264731060
Author: WILLEY
Publisher: MCG
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Chapter 22, Problem 4AL
Summary Introduction

Thiobacillus is called colorless sulfur bacteria because sulfur-containing compounds are used as electron and energy sources. They are sulfur-oxidizing bacteria. Whereas Desulfovibrio is sulfate or sulfur reducing bacteria and during anaerobic respiration, they reduce sulfate or elemental sulfur to hydrogen sulfide.

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In less than 100 words total i) describe the physiological reaction of the red population to alcohol consumption, which is usually not seen with the blue population, and ii) explain in the language of this unit how the enzymatic properties of aldehyde dehydrogenase relate to this physiological reaction.
i) Re-arrange the Michaelis Menten equation so it involves the ratio [S]. Show all steps beginning Km noting any assumptions or required conditions. Km ii) Calculate the ratio [lo for the case when the rate of product formation is 68% of Vmax and the substrate is in great excess. d[P] dt : k₂ with = [E],[S] Km+[S]' [S]o Km iii) Explain, in a few sentences, why the ratio determines the ratio V Vmax V Vmax Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient. Include in your explanation the factors that effect v and Vmax. Consider what factors make v different from or equal to Vmax. Consider what Km represents concerning processes involving ES. " iv) Calculate KM at 310K at given the following rate constant information: k₁ = 17 s-¹M-1 at 300K with A = 7300 s-¹M-1 K-1₁ 6 s¹ at 300K with A = 14500 s -1 k₂ = 31 s¹ at 300K with A = 600 s-¹
Arsenate is a compound that inhibits substrate-level phosphorylation. Compare the effect of this compound on an H2-oxidizing chemolithotroph, on a sulfite-oxidizing chemolithotroph, and on a chemoorganotroph carrying out fermentation.

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PRESCOTT'S MICROBIO W/PROCTORIO

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