Concept explainers
Determine the amino acid sequence of a polypeptide from the following data:
Complete hydrolysis of the peptide yields Ala, Arg, Gly, 2 Lys, Met, Phe, Pro, 2 Ser, Tyr, and Val, Treatment with Edman's reagent releases PTH-Val.
Carboxypeptidase A releases Ala.
Treatment with cyanogen bromide yields the following two peptides:
1. Ala, 2 Lys, Phe, Pro, Ser, Tyr
2. Arg, Gly, Met, Ser, Val
Treatment with trypsin yields the following three peptides:
l. Gly, Lys, Met, Tyr
2. Ala, Lys, Phe, Pro, Ser
3. Arg, Ser, Val
Treatment with chymotrypsin yields the following three peptides:
1. 2 Lys, Phe, Pro
2. Arg, Gly, Met, Ser, Tyr, Val
3. Ala, Ser
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Organic Chemistry, Books a la Carte Edition and Study Guide and Student's Solutions Manual for Organic Chemistry (7th Edition)
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