The reason for why mannose is not formed in the enzyme catalyzed reaction and mannose is one of the products when glucose undergoes base-catalyzed isomerization in the absence of a catalyst has to be explained Concept introduction: Enzyme is a protein that is a biological catalyst. The reactant in a reaction catalyzed by the enzyme is called substrate. In the enzyme, a pocket like cleft is present called active site where it binds the substrate. Some amino acid chains in the enzyme act as base, acid and nucleophilic catalyst. Many of the enzymes contain metal ions at the active site which acts as catalyst. Acid Catalyst helps in increasing the rate of a particular reaction by the donation of a proton to substrate. Base Catalyst helps in increasing the rate of a particular reaction by the removal of a proton to substrate. Nucleophilic catalysis helps in increasing the rate of a particular reaction by the formation of a covalent bond with substrate Glycolysis: A series of reactions where glucose is converted to two pyruvate molecules in the presence of enzymes catalysis.

Question

If you take crystals of the disaccharide lactose the natural substrate for beta-galactosidase and drop them in water, why doesn’t the lactose immediately react with the water and come apart into glucose and galactose?

Answer 1

Question

Chapter 23.11, Problem 22P

Interpretation Introduction

Interpretation: The reason for why mannose is not formed in the enzyme catalyzed reaction and mannose is one of the products when glucose undergoes base-catalyzed isomerization in the absence of a catalyst has to be explained

Concept introduction:

Enzyme is a protein that is a biological catalyst. The reactant in a reaction catalyzed by the enzyme is called substrate. In the enzyme, a pocket like cleft is present called active site where it binds the substrate.

Some amino acid chains in the enzyme act as base, acid and nucleophilic catalyst. Many of the enzymes contain metal ions at the active site which acts as catalyst.

Acid Catalyst helps in increasing the rate of a particular reaction by the donation of a proton to substrate.

Base Catalyst helps in increasing the rate of a particular reaction by the removal of a proton to substrate.

Nucleophilic catalysis helps in increasing the rate of a particular reaction by the formation of a covalent bond with substrate

Glycolysis: A series of reactions where glucose is converted to two pyruvate molecules in the presence of enzymes catalysis.

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