The heptapeptide sequence of amino acids, on the basis of the information provided, is to be deduced. Concept introduction: On treating a polypeptide with 2,4-dinitrofluorobenzene in basic solution, the free amino group of the N-terminal gives a nucleophilic aromatic substitution reaction, which, on hydrolysis, gives a mixture of amino acids in which the N-terminal amino acid is labeled with a 2,4-dinitrophenyl group. The C-terminal residues are identified through the carboxypeptidase enzymes. These enzymes catalyze the hydrolysis of the amide bond of the amino acid residue containing a free liberating carboxylic acid group.

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Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 24, Problem 23P

Interpretation Introduction

Interpretation:

The heptapeptide sequence of amino acids, on the basis of the information provided, is to be deduced.

Concept introduction:

On treating a polypeptide with 2,4-dinitrofluorobenzene in basic solution, the free amino group of the N-terminal gives a nucleophilic aromatic substitution reaction, which, on hydrolysis, gives a mixture of amino acids in which the N-terminal amino acid is labeled with a 2,4-dinitrophenyl group. The C-terminal residues are identified through the carboxypeptidase enzymes. These enzymes catalyze the hydrolysis of the amide bond of the amino acid residue containing a free liberating carboxylic acid group.

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Determine the amino acid sequence of a polypeptide from the following data: Acid-catalyzed hydrolysis gives Ala, Arg, His, 2 Lys, Leu, 2 Met, Pro, 2 Ser, Thr, and Val. Carboxypeptidase A releases Val. Edman’s reagent releases PTH-Leu. Treatment with cyanogen bromide gives three peptides with the following amino acid compositions: 1. His, Lys, Met, Pro, Ser 2. Thr, Val 3. Ala, Arg, Leu, Lys, Met, Ser Trypsin-catalyzed hydrolysis gives three peptides and a single amino acid: 1. Arg, Leu, Ser 2. Met, Pro, Ser, Thr, Val 3. Lys 4. Ala, His, Lys, Met

Determine the amino acid sequence of a polypeptide from the following data:Acid-catalyzed hydrolysis gives Ala, Arg, His, 2 Lys, Leu, 2 Met, Pro, 2 Ser, Thr, and Val.Carboxypeptidase A releases Val.Edman’s reagent releases PTH-Leu.Treatment with cyanogen bromide gives three peptides with the following amino acid compositions:1. His, Lys, Met, Pro, Ser 2. Thr, Val 3. Ala, Arg, Leu, Lys, Met, Ser

Determine the primary structure of an octapeptide from the following data: Acid-catalyzed hydrolysis gives 2 Arg, Leu, Lys, Met, Phe, Ser, and Tyr. Carboxypeptidase A releases Ser. Edman’s reagent releases Leu. Treatment with cyanogen bromide forms two peptides with the following amino acid compositions: 1. Arg, Phe, Ser 2. Arg, Leu, Lys, Met, Tyr Trypsin-catalyzed hydrolysis forms the following two amino acids and two peptides: 1. Arg 2. Ser 3. Arg, Met, Phe 4. Leu, Lys, Tyr

Answer 2

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Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 24, Problem 23P

Interpretation Introduction

Interpretation:

The heptapeptide sequence of amino acids, on the basis of the information provided, is to be deduced.

Concept introduction:

On treating a polypeptide with 2,4-dinitrofluorobenzene in basic solution, the free amino group of the N-terminal gives a nucleophilic aromatic substitution reaction, which, on hydrolysis, gives a mixture of amino acids in which the N-terminal amino acid is labeled with a 2,4-dinitrophenyl group. The C-terminal residues are identified through the carboxypeptidase enzymes. These enzymes catalyze the hydrolysis of the amide bond of the amino acid residue containing a free liberating carboxylic acid group.

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Answer 3

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 24, Problem 23P

Interpretation Introduction

Interpretation:

The heptapeptide sequence of amino acids, on the basis of the information provided, is to be deduced.

Concept introduction:

On treating a polypeptide with 2,4-dinitrofluorobenzene in basic solution, the free amino group of the N-terminal gives a nucleophilic aromatic substitution reaction, which, on hydrolysis, gives a mixture of amino acids in which the N-terminal amino acid is labeled with a 2,4-dinitrophenyl group. The C-terminal residues are identified through the carboxypeptidase enzymes. These enzymes catalyze the hydrolysis of the amide bond of the amino acid residue containing a free liberating carboxylic acid group.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 24, Problem 23P

Interpretation Introduction