Introduction to General, Organic a...

11th Edition
Frederick A. Bettelheim + 4 others
Publisher: Cengage Learning
ISBN: 9781285869759



Introduction to General, Organic a...

11th Edition
Frederick A. Bettelheim + 4 others
Publisher: Cengage Learning
ISBN: 9781285869759
Chapter 26, Problem 26.100P
Textbook Problem

-100 A new endonuclease is found. It cleaves double-

stranded DNA at every location where C and G

are paired on opposite strands. Could this enzyme

be used in producing human insulin by the recom-

binant DNA technique? Explain.

Interpretation Introduction


Whether a new endonuclease enzyme can be used in producing human insulin by the recombinant DNA technique is to be predicted and an explanation for the same is to be stated.

Concept Introduction:

The full form of DNA is Deoxyribose Nucleic Acid. It contains nitrogenous bases, phosphate and ribose sugar. The molecule of DNA is wrapped by histone. Histone is a protein which is made of amino acids. DNA acts as hereditary material due to which the specific characters of parents transfer to their offsprings.

Explanation of Solution

The endonuclease enzymes cleave at particular sites in the DNA. DNA molecule is composed of two chains. The cleavage of DNA by endonuclease enzyme results in the formation of two sticky ends. The ligase enzyme acts on these sticky ends with another DNA molecule to produce a new molecule of DNA.

In recombinant DNA technique, the two DNA molecules which are taken from two different species combine to form a new molecule of DNA.

It is given that endonuclease cleaves double-stranded DNA where C and G are paired on opposite strands...

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Chapter 26 Solutions

Introduction to General, Organic and Biochemistry
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Ch. 26 - Which end of the DNA contains the termination...Ch. 26 - What would happen to the transcription process if...Ch. 26 - Where is the methyl group located in the guanine...Ch. 26 - How are the adenine nucleotides linked together in...Ch. 26 - What is the difference in the requirement for a...Ch. 26 - What are the different names used for the two...Ch. 26 - What is a consensus sequence in transcription?Ch. 26 - What is a promoter sequence?Ch. 26 - What is an intron?Ch. 26 - What is an exon?Ch. 26 - Where are the codons located?Ch. 26 - What are the two most important sites on tRNA...Ch. 26 - What are the ribosomal subunits for eukaryotic...Ch. 26 - (a) If a codon is GCU, what is the anticodon? (b)...Ch. 26 - If a segment of DNA is 981 units long, how many...Ch. 26 - In what sense does the universality of the genetic...Ch. 26 - Which amino acids have the most possible codons?...Ch. 26 - Using the first column of Table 26-1, explain how...Ch. 26 - A genetic code in which two bases encode a single...Ch. 26 - What is meant by the genetic code being continuous...Ch. 26 - To which end of the tRNA is the amino acid bonded?...Ch. 26 - There are three sites on the ribosome, each...Ch. 26 - What is the main role Of (a) the 40S ribosome and...Ch. 26 - What are the prokaryotic equivalents Of the...Ch. 26 - What is the function of elongation proteins?Ch. 26 - What are the stages of protein synthesis?Ch. 26 - Explain the nature Of the tRNA used to initiate...Ch. 26 - Explain what happens to the fMet initially put at...Ch. 26 - Explain why scientists now refer to the ribosome...Ch. 26 - Why is amino acid activation called the second...Ch. 26 - Which molecules are involved in gene regulation at...Ch. 26 - Where are enhancers located? How do they work?Ch. 26 - Where are the sieving portions of AARS enzymes...Ch. 26 - What are the two types Of transcription factors,...Ch. 26 - What is the difference between an enhancer and a...Ch. 26 - How does alternative splicing lead to protein...Ch. 26 - What is the function of proteosomes in quality...Ch. 26 - What kind of interactions exist between metal-...Ch. 26 - Using Table 26-1, give an example of a mutation...Ch. 26 - 26-50 How do cells repair mutations caused by...Ch. 26 - Can a harmful mutation-causing genetic disease ex-...Ch. 26 - Are all mutagens also carcinogens?Ch. 26 - 26-53 How do restriction endonucleases operate?Ch. 26 - 26-54 What are sticky ends?Ch. 26 - A new genetically engineered corn has been...Ch. 26 - EcoRl restriction endonuclease recognizes the...Ch. 26 - Why can it be argued that the discovery Of...Ch. 26 - 26-58 What is gene therapy?Ch. 26 - What disease has been most successfully treated by...Ch. 26 - What does "ex vivo" mean in reference to gene...Ch. 26 - Why are the vectors used for ex vivo gene therapy...Ch. 26 - What does "in vivo" mean in reference to gene...Ch. 26 - Why has human gene therapy only been approved for...Ch. 26 - What is an epigenetic change?Ch. 26 - What are common epigenetic changes?Ch. 26 - What is chromatin remodeling?Ch. 26 - 26-67 How are epigenetic change related to...Ch. 26 - (Chemical Connections 26A) Why is selenocysteine...Ch. 26 - (Chemical Connections 26B) What process distin-...Ch. 26 - (Chemical Connections 26B) What transcription...Ch. 26 - (Chemical Connections 26B) What is one experiment...Ch. 26 - (Chemical Connections 26B) How does the strength...Ch. 26 - (Chemical Connections 26C) What is an in variant...Ch. 26 - (Chemical Connections 26D) What is a silent...Ch. 26 - (Chemical Connections 26D) If an mRNA codon has...Ch. 26 - (Chemical Connections 26D) If an mRNA codon has...Ch. 26 - (Chemical Connections 26D) Why can a silent muta-...Ch. 26 - (Chemical Connections 26D) How was the gene MDRI...Ch. 26 - (Chemical Connections 26E) What is p53? Why is its...Ch. 26 - (Chemical Connections 26E) How does p53 promote...Ch. 26 - (Chemical Connections 26F) Why was cystic fibrosis...Ch. 26 - (Chemical Connections 26F) What is the cause Of...Ch. 26 - (Chemical Connections 26F) Why was it a major...Ch. 26 - (Chemical Connections 26F) Why did the first...Ch. 26 - (Chemical Connections 26F) How do the two drugs by...Ch. 26 - (Chemical Connections 26F) Describe the most...Ch. 26 - (Chemical Connections 26G) What is an epimutation?Ch. 26 - (Chemical Connections 26G) What is the link be-...Ch. 26 - (Chemical Connections 26G) How did scientists re-...Ch. 26 - (Chemical Connections 26G) What is the epigenome?Ch. 26 - In both the transcription and the translation...Ch. 26 - In the tRNA structure, there are stretches where...Ch. 26 - Is there any way to prevent a hereditary disease?...Ch. 26 - How does the cell ensure that a specific amino...Ch. 26 - (a) What is a plasmid? (b) How does it differ from...Ch. 26 - Why do we call the genetic code degenerate?Ch. 26 - Glycine, alanine, and valine are classified as...Ch. 26 - Looking at the multiplicity (degeneracy) of the...Ch. 26 - Which polypeptide is coded for by the mRNA...Ch. 26 - -100 A new endonuclease is found. It cleaves...

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