a) I-L-P-F Interpretation: The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F is to be given. Concept introduction: Edmund degradation involves treatment of a peptide with phenyl isothiocyanate, followed by reaction with trifluoroacetic acid when an anilinothiazolinone (ATZ) is produced. This ATZ rearranges in the presence of acids to yield N-phenylthiohydantoin. To give: The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F.

Question

An unknown decapeptide was isolated and characterized. Complete hydrolysis of this peptide gave : F(2), A,G,C,K,N,T, W and V. Treatment with carboxypeptidase releases A. Reaction with Edman’s reagent gave PTH-T and a nonapeptide. The nonapeptide was treated with trypsin and gave 2 peptides: (V-C-G-A) and (N-FF-W-K). Give the sequence of amino acid in the decapeptide.

Answer 1

Question

Chapter 26.SE, Problem 45AP

Interpretation Introduction

a) I-L-P-F

Interpretation:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F is to be given.

Concept introduction:

Edmund degradation involves treatment of a peptide with phenyl isothiocyanate, followed by reaction with trifluoroacetic acid when an anilinothiazolinone (ATZ) is produced. This ATZ rearranges in the presence of acids to yield N-phenylthiohydantoin.

To give:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F.

Interpretation Introduction

b) D-T-S-G-A

Interpretation:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide D-T-S-G-A is to be given.

Concept introduction:

Edmund degradation involves treatment of a peptide with phenyl isothiocyanate, followed by reaction with trifluoroacetic acid when an anilinothiazolinone (ATZ) is produced. This ATZ rearranges in the presence of acids to yield N-phenylthiohydantoin.

To give:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide D-T-S-G-A.

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