Biology with Connect Access Card
10th Edition
ISBN: 9780077705701
Author: Raven, Peter
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 27, Problem 5S
How might phage λ be used to transfer E. coli genes between different bacterial cells? Could this be used to transfer any gene?
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Students have asked these similar questions
What is horizontal gene transfer? What are the three mechanisms for this to occur in bacteria?
What are the components needed for the processes of transformation, conjugation, and transduction? How does each process occur? What genes are involved in each process?
How do generalized and specialized transduction differ? What is the end result of each?
What is recombination? What is the importance to bacteria & archaea?
What are the two types of recombination? What are the details of each type? What components are needed for each type?
What is a transducing phage?
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
Chapter 27 Solutions
Biology with Connect Access Card
Ch. 27 - The reverse transcriptase enzyme is active in...Ch. 27 - Which of the following is NOT part of a virus? a....Ch. 27 - Which of the following is common in animal viruses...Ch. 27 - Which of the following would NOT be part of the...Ch. 27 - A process by which a virus may change a benign...Ch. 27 - Prob. 6UCh. 27 - Prob. 7UCh. 27 - Prob. 1ACh. 27 - The idea of a protein that was an infectious agent...Ch. 27 - Bacterial viruses and animal viruses are similar...
Ch. 27 - Prob. 4ACh. 27 - Phage conversion in which viruses add genes to a...Ch. 27 - According to the prion hypothesis, the infectious...Ch. 27 - Prob. 7ACh. 27 - Prob. 8ACh. 27 - E. coli lysogens derived from infection by phage ...Ch. 27 - Most biologists believe that viruses evolved...Ch. 27 - Prob. 3SCh. 27 - What do we mean by the term emerging virus? How is...Ch. 27 - How might phage be used to transfer E. coli genes...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Why is phage conversion considered beneficial to host cells?arrow_forwardAfter a phage injects its DNA into a bacterial cell, the cell begins making proteins that make up the phage coat. Why does the same thing not happen when a generalized transducing particle injects the DNA it carries?arrow_forwardIs a phage a bacteria?arrow_forward
- What is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance.arrow_forwardIf we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forwardOne of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forward
- When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?arrow_forwardConsider three genes in E. coli: thr+, ara+, and leu+ (which give the cell the ability to synthesize threonine, arabinose, and leucine, respectively). All three of these genes are close together on the E. coli chromosome. Phages are grown in a thr+ ara+ leu+ strain of bacteria (the donor strain). The phage lysate is collected and used to infect a strain of bacteria that is thr− ara− leu −. The recipient bacteria are then tested on selective medium lacking leucine. Bacteria that grow and form colonies on this medium (leu+ transductants) are then replica-plated on medium lacking threonine and on medium lacking arabinose to see which are thr+ and which are ara+. Another group of the recipient bacteria are tested on medium lackingthreonine. Bacteria that grow and form colonies on this medium (thr+ transductants) are then replica-plated on medium lacking leucine and onto medium lacking arabinose to see which are ara+ and which are leu+. Results from these experiments are as follows:…arrow_forwardIn a P1 transduction experiment, the P1 lysate contains phages thatcarry pieces of the host chromosomal DNA, but the lysate also containsbroken pieces of chromosomal DNA. If a P1 lysate is used to transfer chromosomal DNA to another bacterium, how could you show experimentally that the recombinant bacterium has been transduced (i.e., has taken up a P1 phage with a piece of chromosomal DNA inside) versus transformed (i.e., has taken up a piece of chromosomal DNA that is not within a P1 phage coat)?arrow_forward
- What is pGLO plasmid? How has it been engineered so that the cells that carry it can be detected? Attach a map of it.?arrow_forwardWhen would it be beneficial to use a eukaryotic host formolecular cloning?arrow_forwardIn which of the four processes shown can material from a bacterial genome be transferred from one cell to another? Which are forms of horizontal transmission? Place the answer(s) in the cell highlighting that process. Partial genome transfer by DNA uptake Transformation Answer Bank material from a bacterial genome horizontal gene transfer Conjugation Plasmids Conjugation Genome Plasmid transfer during conjugation Partial genome transfer during conjugation Virus Transduction Transfer as part of viral genomearrow_forward
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