Brock Biology of Microorganisms Plus Mastering Microbiology with Pearson eText -- Access Card Package (15th Edition)
15th Edition
ISBN: 9780134268668
Author: MADIGAN
Publisher: PEARSON
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Chapter 27.2, Problem 1CR
Q What properties are required for a vaccine to induce an immune response? What type of immunity results from vaccination, and what role does a “booster” play in this process?
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Q. Only Somatic Diversification Theory can explain the huge repertoire of Antibodies against millions of antigens.
1) You have been asked to engineer a protein (which is not animmunoglobulin) that is capable of binding to a given protein target.Devise a strategy and discuss the main molecular screeningapproaches required.
MCQ.
It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate?
a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody
b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies
c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies
d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
Chapter 27 Solutions
Brock Biology of Microorganisms Plus Mastering Microbiology with Pearson eText -- Access Card Package (15th Edition)
Ch. 27.1 - Prob. 1MQCh. 27.1 - Prob. 2MQCh. 27.1 - Distinguish between clonal deletion and clonal...Ch. 27.1 - QWhy is it necessary that all three defining...Ch. 27.2 - Identify the intrinsic and extrinsic properties of...Ch. 27.2 - Describe an epitope recognized by an antibody, and...Ch. 27.2 - Give an example for each: natural and artificial...Ch. 27.2 - QWhat properties are required for a vaccine to...Ch. 27.3 - Summarize antibody production starting with...Ch. 27.3 - Differentiate among antibody classes using...
Ch. 27.3 - Prob. 3MQCh. 27.3 - QDescribe the structural and functional...Ch. 27.4 - Draw a complete Ig molecule and identify...Ch. 27.4 - Describe antigen binding to the CDR1, 2, and 3...Ch. 27.4 - Describe the recombination events that produce a...Ch. 27.4 - QWhich Ig chains are used to construct a complete...Ch. 27.5 - Identify the cells that display MHC class I and...Ch. 27.5 - Compare the MHC I and MHC II protein structures...Ch. 27.5 - Define the sequence of events for processing and...Ch. 27.5 - QDescribe the basic structure of class I and class...Ch. 27.6 - Define polymorphism and polygeny as they apply to...Ch. 27.6 - How does a single MHC protein present many...Ch. 27.6 - QPolymorphism implies that each different MHC...Ch. 27.7 - Prob. 1MQCh. 27.7 - Identify diversity-generating mechanisms unique to...Ch. 27.7 - Describe and compare the structural features of Ig...Ch. 27.7 - QWhat diversity-generating mechanisms function to...Ch. 27.8 - Describe the mechanism used by Tc cells to...Ch. 27.8 - Describe the effector system (the cell-killing...Ch. 27.8 - Compare and contrast the roles and activities of...Ch. 27.8 - QWhat mechanism do Tc cells use to identify and...Ch. 27.9 - Discriminate between immediate hypersensitivity...Ch. 27.9 - Provide examples and mechanisms for an...Ch. 27.9 - QHow do immediate and delayed-type...Ch. 27.10 - Describe the binding site for superantigens on T...Ch. 27.10 - Compare and contrast the immunodeficiency observed...Ch. 27.10 - Prob. 3MQCh. 27.10 - Prob. 1CRCh. 27 - Antibodies of the IgA class are probably more...Ch. 27 - Prob. 2AQCh. 27 - Polymorphism implies that each different MHC...Ch. 27 - What problems would arise if a person had a...
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- Briefly explain antigenic drift and antigen shift. Which is usually more dangerous of the twoand why? How do they affect production of vaccines?arrow_forward24. Match the following types of vaccines with the active antigen each includes. Each antigen is only used once. live attenuated vaccine inactivated vaccine subunit vaccine toxoid vaccine DNA vaccine A. inactivated bacterial toxin B. weakened strain of a pathogen C. killed strain of a pathogen D. piece of nucleic acid E. antigensarrow_forward2. Describe the technique of immunocytochemistry and give an example of a specific cellular component it could help visualize. Why is it important to know the species in which a primary antibody is generated? What might happen if you were using a primary antibody generated in mice to visualize a mouse protein?arrow_forward
- Smallpox has been widely reported as a possible bioterror weapon. Given what you know about the etiology of the disease and the current state of the world’s immunity to smallpox, discuss how effective (or ineffective) a smallpox weapon might be. What kind of defense could be mounted against such an attack?arrow_forwardDescribe the preparation of killed vaccines; live, attenuatedvaccines;subunit vaccines; recombinant vaccines; and DNAvaccines.Explain the advantages and disadvantages ofarrow_forwardMortality rate induced by Covid 19 will be reduced when vaccinated.... what does this mean?arrow_forward
- 1aDiscuss three differences between antigen and antibodies b Explain the structure of the antibody molecule. c Discuss the differences between humoral and cell mediated immunity in terms of chemicals and cells involved in each process as they tackle pathogens.arrow_forward3. Explain in detail why COVID-19 became pandemic, and SARS did not despite both being caused by very similar pathogens? Give typing answer with explanation and conclusionarrow_forward5) In the event of an inflammation in the body, discuss the vaccine concept after stating what the proteins that cause this formation in the cell where the inflammation takes place and consequently cause the initiation of the fight against this foreign substance.arrow_forward
- Describe IN DETAIL how vaccines work. Include a clear description of how the immune system works and how vaccines work WITH our natural immune function. How do they help protect us from dying from diseases? Use examples from the past (e.g. polio, smallpox, measles, rubella, etc.) to illustrate the effectiveness of vaccines.arrow_forwardOur immune system is successfully elicited by the Coronavirus. Instruction: Differentiate the two: Antigen vs Immunogen. 1. What are the viral properties of Coronavirus? What does it look like? (Provide a drawing/sketch) 2. Give a brief lab report about the properties of Coronavirus as an immunogen.arrow_forwardIf antibody responses are not elicited by a Covid-19 vaccine, are there other types of immune response that could provide protection from the SARS-Co-V2 virus following vaccination? Explain why or why not.arrow_forward
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