Bio 121 Campbell Biology Truman College
17th Edition
ISBN: 9781323670637
Author: Urry, Cain
Publisher: PEARSON
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Chapter 27.2, Problem 2CC
Distinguish between the three mechanisms by which bac- teria can transfer DNA from one bacterial cell to another.
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Describe how an Hfr strain can transfer portions of the bacterial chromosome to recipient cells?
Describe the three different methods of horizontal gene transfer among bacteria and mention their significance. Be specific when discussing the donor versus recipient cell, and if the donor and recipient cells are still alive after each horizontal gene transfer event is complete.
Please be detailed.
With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it established that physical contact between cells was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis for a bacterium’s being F+?
Chapter 27 Solutions
Bio 121 Campbell Biology Truman College
Ch. 27.1 - Describe two adaptations that enable prokaryotes...Ch. 27.1 - Contrast the cellular and DNA structures of...Ch. 27.1 - Prob. 3CCCh. 27.2 - Although rare on a per gene basis, new mutations...Ch. 27.2 - Distinguish between the three mechanisms by which...Ch. 27.2 - In a rapidly changing environment, which bacterial...Ch. 27.2 - WHAT IF? If a nonpathogenic bacterium were to...Ch. 27.3 - Distinguish between the four major modes of...Ch. 27.3 - A bacterium requires only the amino acid...Ch. 27.3 - WHAT IF? Describe what you might eat for a...
Ch. 27.4 - Explain how molecular systematics and metagenomics...Ch. 27.4 - WHAT IF What would the discovery of a bacterial...Ch. 27.5 - Explain how prokaryotes, though small, can be...Ch. 27.5 - MAKE CONNECTIONS Review Figure 10.6. Then...Ch. 27.6 - Identify at least two ways that prokaryotes have...Ch. 27.6 - Prob. 2CCCh. 27.6 - Prob. 3CCCh. 27 - Describe features of prokaryotes that enable them...Ch. 27 - Mutations are rare and prokaryotes reproduce...Ch. 27 - Describe the range of prokaryotic metabolic...Ch. 27 - How have molecular data informed prokaryotic...Ch. 27 - In what ways are prokaryotes key to the survivaI...Ch. 27 - Prob. 27.6CRCh. 27 - Genetic variation in bacterial populations cannot...Ch. 27 - Photoautotrophs use (A) light as an energy source...Ch. 27 - Which of the following statements is not true? (A)...Ch. 27 - Prob. 4TYUCh. 27 - Prob. 5TYUCh. 27 - Prob. 6TYUCh. 27 - Prob. 7TYUCh. 27 - SCIENTIFIC INQUIRY INTERPRET THE DATA The...Ch. 27 - WRITE ABOUT A THEME: ENERGY In a short essay...Ch. 27 - SYNTHESIZE YOUR KNOWLEDGE Explain how the small...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Identify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culturearrow_forwardOutline the sequence of events that compromise the mechanism used by a bacteriophage in order to infect a bacteria cell?arrow_forwardexplain three methods of genetic transfer in bacteria?arrow_forward
- Describe the 3 ways in which bacteria can transfer genetic material to each other via horizontal gene transfer. Be sure to explain the following: How is DNA transferred between bacteria, is there direct contact between bacteria and whether a plasmid is involved or not.arrow_forwardExplain the following statement : a) initiation of bacteriall DNA replication is an energy requiring process b) bacterial DNA polymerase can enter the termination sequence but cannot exisarrow_forwardIf we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forward
- We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardAssume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transmitted via conjugation, in the other case it is it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it likely that the transferred DNA will be present? left and can function in the recipient cells? Explain the biological background to your answerarrow_forwardMark the alternative that best explains the strategies for using tails and/or fusion proteins associated with the protein of interest. * a) fusion proteins have different functions, including increasing the molecular weight of the protein of interest and thereby facilitating the process of protein purification by affinity chromatography. b) Histidine, c-Myc and GST tails have a dual function, since they can act both in reducing the formation of inclusion bodies and in facilitating the solubility of the protein of interest. c) the introduction of fusion proteins, such as Maltose-binding protein (MBP), at the C-terminus of the protein of interest has as main objective to increase the expression yield as they facilitate the protein aggregation process. d) the introduction of tails, such as c-Myc, in the C-terminus aims to facilitate the protein purification process through the affinity chromatography methodology.arrow_forward
- When an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a recipient E. coli cell, without the use of naked DNA in solution or of a viral vector, this is: an example of horizontal gene transfer by means of lysogenic bacteriophages an example of horizontal gene transfer by means of lytic bacteriophages an example of horizontal gene transfer by means of transformation an example of horizontal gene transfer by means of transduction an example of horizontal gene transfer by means of conjugationarrow_forwardCompare and contrast the three mechanisms of genetic transfer in bacteria?arrow_forwardCompare the three mechanisms of gene transfer in bacteria: transformation, conjugation, and transduction.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license