Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Textbook Question
Chapter 28, Problem 1CAE
Suppose you are culturing a microorganism that produces enough lactic acid to kill itself in a few days.
- a. How can the use of a bioreactor help you maintain the culture for weeks or months? The graph below shows conditions in the bioreactor:
- b. If your desired product is a secondary metabolite, when can you begin collecting it?
- c. If your desired product is the cells themselves and you want to maintain a continuous culture, when can you begin harvesting?
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If a biotechnologist wishes to obtain a primary metabolite, they will harvest the organism/media during log phase. If a biotechnologist wishes to obtain a secondary metabolite, they will harvest the organism/media during stationary phase. Can you explain this distinction regarding the metabolism of the cell at these two different batch culture growth phases?
Why is the sterile technique (the flame sterilization of wire loop) followed during the fermentation lab simulation?
A.
To ensure mono cultivation of yeast cells.
B.
To ensure optimal pH for the yeast cells to grow.
C.
To remove unwanted compounds, such as ammonia, in the medium.
D.
To remove oxygen and maintain anaerobic conditions.
Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours. Which of the following can be interpreted from this result?
A.
The organism did not grow so lysine decarboxylase activity could not be determined.
B.
The organism is negative for lysine decarboxylase.
C.
The media is too acidic to determine lysine decarboxylase activity.
D.
The organism is positive for lysine decarboxylase
Chapter 28 Solutions
Microbiology: An Introduction
Ch. 28 - What is industrial microbiology? Why is it...Ch. 28 - How does commercial sterilization differ from...Ch. 28 - Why is a can of blackberries preserved by...Ch. 28 - Outline the steps in the production of cheese, and...Ch. 28 - Beer is made with water, malt, and yeast; hops are...Ch. 28 - Why is a bioreactor better than a large flask for...Ch. 28 - The manufacture of paper includes the use of...Ch. 28 - Describe an example of bioconversion. What...Ch. 28 - Prob. 9RCh. 28 - NAME IT Van Leeuwenhoek was the first to see this...
Ch. 28 - Which bacteria seem to be most frequently used in...Ch. 28 - Methylophilus methylotrophus can convert methane...Ch. 28 - Faded worn-look denim is produced with cellulase....Ch. 28 - Foods packed in plastic for microwaving are a....Ch. 28 - Acetobacter is necessary for only one of the steps...Ch. 28 - Use the following choices to answer questions 35:...Ch. 28 - The spoilage of canned foods caused by Geobacillus...Ch. 28 - A heat-resistant fungus that causes spoilage in...Ch. 28 - The term 12D treatment refers to a. heat treatment...Ch. 28 - Which one of the following is not a fuel produced...Ch. 28 - Which type of radiation is used to preserve foods?...Ch. 28 - Which of the following reactions is undesirable in...Ch. 28 - Which of the following reactions is an oxidation...Ch. 28 - Suppose you are culturing a microorganism that...Ch. 28 - Researchers at the CDC inoculated apple cider with...Ch. 28 - The antibiotic efrotomycin is produced by...
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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY