Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 28, Problem 2Q
Summary Introduction
To determine:
The reason why the complete and minimal nutrient replica plated.
Introduction:
Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.
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Laboratory Experiments in Microbiology (11th Edition)
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- Why Touch-down PCR technique is better than the Gradient PCR technique? Also, from those techniques which technique produces the pure product?arrow_forwardWhy can we use temperature sensitive genetic screening to identify particular mutants within a sample?arrow_forwardWhat is the rationale for the salting out technique for DNA extraction?arrow_forward
- What is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in a method of identifying recombinant vectors that contain segments of chromosomal DNA.arrow_forwardWhy is it difficult in a single experiment to transfer a largenumber of genes to a recipient cell using transformation ortransduction?arrow_forwardYou can carry out matings between an Hfr and F strain by mixing the two cell types in a small patch on a plate and then replica plating to selective medium. This methodology was used to screen hundreds of different cells for a recombination-deficient recA - mutant. Why is this an assay for RecA function? Would you be screening for a recA mutation in the F or Hfr strain using this protocol? Explain.arrow_forward
- In Colony Polymerase Chain Rxn (PCR), what primers are necessary to identify that the transformants contain the gene of interest with the correct orientation?arrow_forwardwhat is Inverse PCR ?arrow_forwardAssume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.arrow_forward
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