To explain: Why dense or thick smears less likely to provide a good preparation of smear for microscopic evaluation.
Introduction: Smear preparation is needed for several laboratory techniques. The major advantages of preparing a smear are to fix bacteria on the slide and to avoid bacterial loss during a staining technique. A smear can be prepared from a broth or solid medium.
Explanation of Solution
Proper care should be taken while preparing a smear for microscopic examination. The advantages of preparing thin smears are as follows:
1. Thin smears permit easy passage of light, which gives better resolution of bacteria, particularly when using oil immersion.
2. Dense or thick smears will lead to uneven spread of bacteria in the microscopic slide.
3. In the case of dense smears, stains are unable to penetrate the bacterial cell wall.
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