Chapter 3, Problem 9P

Calculate the specific absorbance ratio (SAR), where A620 = 0.136 and A280 = 1.392. Enter your result with 3 decimal places.

If the concentration of Sn2+ ions in a solution of Sn(OH)2 was measured to be (1.60x10^-3), what is the Ksp value for Sn(OH)2? Note: Your answer is assumed to be reduced to the highest power possible.

An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.)

4c. Calculate the values of Mn, Mw, Mz and polydispersity index for a polymer mixture having equivalent moles of two polymers with exact molecular weight being 2,000 Dalton and 10,000 Dalton.

Calculate % transmittance if 85% of light was absorbed by an analyte sample in an UV-Vis spetrophotometric experiment

The absorbances of solutions containing K2CrO4 in 0.05 M KOH were measured in a 1.0-cm cell of 375 nm. The following results were obtained: Calculate the linear equation of the calibration curve.

The molar absorptivity for Blue #1 dye is 1.30105 M‒1 ·cm‒1 at 629 nm. Calculate the expected absorbance of solution of Blue #1 with a concentration of 4.50×10–6M when measured at 629 nm in a 1.50 cm cell.

5.4.5 Chemical microanalysis5.4.5.1 Exploitation of characteristic X-raysElectron probe microanalysis (EPMA) of bulk samples is now a routine technique for obtaining rapid, accurate analysis of alloys. A small electron probe (100 nm diameter) is used to generate X-rays from a defined area of a polished specimen and the intensity of the various characteristic X-rays measured usingeither wavelength-dispersive spectrometers (WDS) or energy-dispersive spectrometers (EDS). Typically the accuracy of the analysis is š0.1%. One of the limitations of EPMA of bulk samples is that the volume of the sample which contributes to the X-ray signal is relatively independent of the size of theelectron probe, because high-angle elastic scattering of electrons within the sample generates X-rays. The consequence of this is that the spatial resolution of EPMA is no better than ¾2 µm. In the last few years EDX detectors have been interfaced totransmission electron microscopes which are capable of operating…

The absorbance of a 1.05x10-5 M Fe+3 determined at 580 nm using a 2.50-cm cell was said to be 0.200. What is the molar absorptivity of this solution in liters/mole cm?