LIU-BKLYN BIO 101 BENSON'S MICROBIOLOGI
LIU-BKLYN BIO 101 BENSON'S MICROBIOLOGI
14th Edition
ISBN: 9781264066940
Author: Brown
Publisher: MCG
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Chapter 46, Problem 1SAQ
Summary Introduction

To explain: The reason why the tubes used for the reductase test is sealed with a rubber stopper rather than being left open.

Introduction: The methylene blue reduction test is due to the fact that the color exerted on milk by adding a dye such as methylene blue would disappear more or less quickly. The formation of reducing substances as well as the removal of oxygen from milk during the bacterial metabolism makes the color to disappear.

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Explanation of Solution

The methylene blue reduction test is dependent on the levels of oxygen present in the milk. This test is conducted using test tubes. When new oxygen from the atmosphere enters the tube, it would affect the test results and therefore, the test tubes would be sealed with a rubber stopper rather than being left open.

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Chapter 5 Microbial Metabolism; Author: Heather Davis;https://www.youtube.com/watch?v=wH_HrsfDWZw;License: Standard Youtube License