Brock Biology of Microorgan. -Access
14th Edition
ISBN: 9780321943736
Author: MADIGAN
Publisher: PEARSON
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Chapter 5, Problem 2AQ
Escherichia coli but not Pyrolobus fumarii will grow at 40°C, while P. fumarii but not E. coli will grow at 110°C. What is happening (or not happening) to prevent growth of each organism at the nonpermissive temperature?
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In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees celsius and under 37 degrees celsius, the number of colonies after doubling time has decreased?
If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli?
In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C
Total time of exposure (minutes):
Number of Microbial Cells Present:
0
100
1
80
3
50
4
42
6.5
26
13
10
21
0
Chapter 5 Solutions
Brock Biology of Microorgan. -Access
Ch. 5.1 - Summarize the steps that lead up to binary fission...Ch. 5.1 - Define the term generation. What is meant by the...Ch. 5.2 - Prob. 1MQCh. 5.2 - Prob. 2MQCh. 5.2 - Prob. 3MQCh. 5.3 - Prob. 1MQCh. 5.3 - Prob. 2MQCh. 5.3 - Prob. 3MQCh. 5.4 - What are autolysins and why are they necessary?
Ch. 5.4 - What is the function of bactoprenol?
Ch. 5.4 - What is transpeptidation and why is it important?
Ch. 5.5 - What is a semilogarithmic plot and what...Ch. 5.5 - Prob. 2MQCh. 5.5 - Prob. 3MQCh. 5.6 - In which phase of the growth curve do cells divide...Ch. 5.6 - Prob. 2MQCh. 5.6 - Prob. 3MQCh. 5.7 - How do microorganisms in a chemostat differ from...Ch. 5.7 - What happens in a chemostat if the dilution rate...Ch. 5.7 - Do pure cultures have to be used in a chemostat?Ch. 5.8 - What are some of the problems that can arise when...Ch. 5.8 - Using microscopic techniques, how could you tell...Ch. 5.9 - Why is a viable count more sensitive than a...Ch. 5.9 - Describe how you would dilute a bacterial culture...Ch. 5.9 - Prob. 3MQCh. 5.10 - List two advantages of using turbidity as a...Ch. 5.10 - Describe how you could use a turbidity measurement...Ch. 5.11 - How does a hyperthermophile differ from a...Ch. 5.11 - Prob. 2MQCh. 5.11 - E. coli can grow at a higher temperature in a...Ch. 5.12 - Prob. 1MQCh. 5.12 - What molecular adaptations to cold temperatures...Ch. 5.13 - Which phylogenetic domain includes species with...Ch. 5.13 - How does the membrane structure of...Ch. 5.13 - What is Taq polymerase and why is it important?Ch. 5.14 - How does the concentration of H+ change when a...Ch. 5.14 - What terms are used to describe organisms whose...Ch. 5.15 - What is the aw of pure water? What is the lower...Ch. 5.15 - What are compatible solutes, and when and why are...Ch. 5.16 - How does an obligate aerobe differ from a...Ch. 5.16 - How does a reducing agent work? Give an example of...Ch. 5.16 - How does Superoxide dismutase or superoxide...Ch. 5.17 - Why is heat an effective sterilizing agent?Ch. 5.17 - What steps are necessary to ensure the sterility...Ch. 5.17 - Distinguish between the sterilization of...Ch. 5.18 - Define D10 and explain why the killing dose for...Ch. 5.18 - Prob. 2MQCh. 5.18 - Prob. 3MQCh. 5.19 - Distinguish between the antimicrobial effects of...Ch. 5.19 - Describe how the minimum inhibitory concentration...Ch. 5.19 - Distinguish between a sterilant, a disinfectant,...Ch. 5 - Prob. 1RQCh. 5 - Describe the role of proteins present at the...Ch. 5 - Prob. 3RQCh. 5 - Describe how new peptidoglycan subunits are...Ch. 5 - Prob. 5RQCh. 5 - Describe the growth cycle of a population of...Ch. 5 - How does a chemostat regulate growth rate and cell...Ch. 5 - How does a viable count differ from a total count?Ch. 5 - How can turbidity be used as a measure of cell...Ch. 5 - Prob. 10RQCh. 5 - Prob. 11RQCh. 5 - Concerning the pH of the environment and of the...Ch. 5 - How does a halophile maintain positive water...Ch. 5 - Prob. 14RQCh. 5 - Prob. 15RQCh. 5 - Contrast the terms thermal death time and decimal...Ch. 5 - Prob. 17RQCh. 5 - Prob. 18RQCh. 5 - Prob. 19RQCh. 5 - Describe the procedure for obtaining the minimum...Ch. 5 - Prob. 21RQCh. 5 - A medium was inoculated with 5 106 cells/ml of...Ch. 5 - Escherichia coli but not Pyrolobus fumarii will...Ch. 5 - In which direction (into or out of the cell) will...
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