Molecular Cell Biology
Molecular Cell Biology
8th Edition
ISBN: 9781464183393
Author: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Kelsey C. Martin
Publisher: W. H. Freeman
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Chapter 5, Problem 2RTC
Summary Introduction

To write:

The increase in temperature to 90°C and then decreasing to 25°C helps in preparing a plasmid for sequencing.

Introduction:

DNA is made of two strands which are held together with the help of hydrogen bonds between the complementary base pairs. For the replication process, these strands need to get separated, in order to generate a new sequence. This is where temperature plays a very important role.

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