Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 6, Problem 1CA
Summary Introduction
To write:
Treponema denticolacan be simply missed in a direct stain of a scraping of gum or tooth because the cells are distorted by the staining procedure. The reason that this bacterium could be seen with negative staining.
Introduction:
Heat fixing:
After the drying of smear at the room temperature on the slide. This slide is gripped with the help of tongs or a clothespin. Then it is passed across the flame of a Bunsen burner and numerous times to heat-kill. It attaches the organism to the slide. Heat fixation usually conserves generally the morphology but not an internal structure.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a gram stain is to be performed on this particular microbe, it often takes the gram stain reaction. Why?
Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
Chapter 6 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Describe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.arrow_forwardWhich of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.arrow_forwardWhy is mordant necessary for the staining of bacterial flagella? Answer in not more than 3 sentences.arrow_forward
- What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forwardAre there any chemical differences between the cell walls of gram-positive and negative bacteria that might explain differences in the rate of decolorization ? Does the age of a culture affect the gram stain reaction ? Why or why not?arrow_forwardWhich of the following media selects for gram negative bacteria by inhibiting the growth of gram positives that typically incorporate its dye component into their relatively bigger cell walls? blood agar EMB Mannitol salt agar Simmon citrate agar urease brotharrow_forward
- What is the purpose of each reagent in gram-staining? Primary stain Mordant Decolorizing agent Counterstainarrow_forwardBacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorizationarrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Gram Stain - Acid fast stainarrow_forward
- The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?arrow_forwardWhen you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus Arrangementarrow_forwardComplete the following table with your observations from the prepared slide Name of the Bacteria Type of Endospore Staining Technique (+ or −) Drawing Clostridium botulinum Clostridium perfringens Bacillus anthracisarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
