Concept explainers
MATHEMATICAL The hydrolysis of a phenylalanine-containing peptide is catalyzed by
Interpretation:
The values of
Concept introduction:
In an enzymatic reaction,
The
Answer to Problem 29RE
The graph shows a straight line and on extrapolating the line, it intersects X-axis as well as Y-axis, and the desired value is determined.
The value of the Y-intercept is 450, which also represents
So, the value of
Conversion of
The value of the X- intercept is -800, which represents the value of
So, the value of
Explanation of Solution
Given information:
The reciprocal of the substrate concentration and the velocity is required to draw the respective plots. Before plotting the graph, the data must be converted. The reaction velocity data is given in mole/min. It needs to be converted into mole/sec by dividing by 60.
In enzyme kinetics, the values of
This equation is further modified so that it can represent in a straight line.
Modification of the equation is,
From this equation, the values of both
With the help of the Lineweaver–Burk plot, the values of both
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Chapter 6 Solutions
EBK BIOCHEMISTRY
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- MATHEMATICAL Consider the reaction AB+C, where G=0.00. (a) What is the value of G (not G) when the initial concentrations of A, B, and C are 1 M, 103M,and106M? (b) Try the same calculations for the reaction D+EF, for the same relative order of concentrations. (c) Try the same calculations for the reaction GH, if the concentrations are 1Mand103M for G and H, respectively.arrow_forwardREFLECT AND APPLY What is the energy cost per amino acid in prokaryotic protein synthesis? Relate this to low entropy.arrow_forwardREFLECT AND APPLLY What are the metabolic effects of not being able to produce the M subunit of phosphofructokinase?arrow_forward
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning