MICROBIOLOGY W/DISEASES (SPIRAL) >CI<
17th Edition
ISBN: 9781323471661
Author: BAUMAN
Publisher: PEARSON C
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 6, Problem 2MC
“In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals.” This statement describes which of the following?
- a. broth culture
- b. pour plate
- c. streak plate
- d. dilution plate
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Which of the following is the proper technique for inoculating an agar slant with a broth culture?
A.
Stab the butt of the media with the wire loop.
B.
Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop.
C.
Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant.
D.
Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
1 ml of a patient sample was added to 999 ml of media. From this 0.1 ml was transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample.
a.
The dilution factor is 1000. The patient sample contains 600 cells/ml
b.
The dilution factor is 1000. The patient sample contains 600,000 cells/ml
c.
The dilution factor is 100. The patient sample contains 600,000 cells/ml
d.
The dilution factor is 10. The patient sample contains 6000 cells/ml
e.
It cant be determined its too many cells
In the conduct of serial dilution, which of the following tools is NOT necessary?
a.
Agar plates
b.
Micropipettor
c.
Inoculating needle
d.
Alcohol lamp or Bunsen burner
e.
Test tubes or microcentrifuge tubes
Chapter 6 Solutions
MICROBIOLOGY W/DISEASES (SPIRAL) >CI<
Ch. 6 - Why should cardiac nurses and respiratory...Ch. 6 - Cavities Gone Wild Five-year-old Daniel appears to...Ch. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Boils in the Locker Room For several weeks,...Ch. 6 - Some students transfer some gunk from a 2-week-old...Ch. 6 - Which of the following can grow in a Petri plate...Ch. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...
Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Which of the following terms best describes an...Ch. 6 - In a defined medium, ______________. a. the exact...Ch. 6 - Which of the following is most useful in...Ch. 6 - Which of the following methods is best for...Ch. 6 - A Coulter counter is a(n) ________________. a....Ch. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Quorum sensing is _____________. a. the ability to...Ch. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Fill in the Blanks 6. Cells that shrink in...Ch. 6 - Fill in the Blanks 7. Obligate ________ exist in...Ch. 6 - Prob. 8FIBCh. 6 - Fill in the Blanks 9. Microbes that reduce N2 to...Ch. 6 - Fill in the Blanks 10. A student observes a...Ch. 6 - Fill in the Blanks 11. Chemolithotrophs acquire...Ch. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Explain quorum sensing, and describe how it is...Ch. 6 - Why must media, vessels, and instruments be...Ch. 6 - Why is agar used in microbiology?Ch. 6 - What is the difference between complex media and...Ch. 6 - Draw and label the four distinct phases of a...Ch. 6 - If there are 47 cells in 1 l of sewage, how many...Ch. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Contrast the media described in Tables 6.3 and 6.4...Ch. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Two cultures of a facultative anaerobe are grown...Ch. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Suppose you perform a serial dilution of 0.1-ml...Ch. 6 - How might the study of biofilms benefit humans?Ch. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Given that Haemophilus ducreyi is a...Ch. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Prob. 19CTCh. 6 - Prob. 20CTCh. 6 - Prob. 21CTCh. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Using the following terms, fill in the following...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is transferred to the wire loop but before transfer to new media B. before culture is transferred to the wire loop and after culture is transferred from the wire loop to new media C. after culture is transferred to the wire loop and before culture is transferred from the wire loop to new media D. before culture is transferred to the wire looparrow_forwardA glass flask is rinsed and autoclaved after use. It was then filled with sterile media in a sterile environment and covered. A few days, microbial growth appears in the flask. Assume the media and sterile environment is guaranteed to be sterile. What most likely caused the growth in the flask? a. The flask has to be rinsed with bleach before autoclaving b. A portion of the flask was not exposed to the steam c. Autoclaves only sanitize equipment, so you cannot expect the flask to be sterile d. Autoclaving inhibits growth, but does not kill microbesarrow_forwardWhich type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infectionarrow_forward
- This agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken from cole slaw, and then incubating at 37oC for 24h. This plate indicates that: a. there are 42 bacteria/ml on the cole slaw b. there are at least 4 different types of bacteria in the cole slaw c. there are approximately 42,000 bacteria/ml in the cole slaw sample d. there are approximately 420,000 bacteria/ml in the cole slaw e. these bacteria cannot cause illness because they came from the person’s skin who made the cole slawarrow_forwardIn the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria placed on the plate b. type of medium in the plate c. concentration of antimicrobial drug in the disk d. all of thesearrow_forwardExplain why the following steps are essential during subculturing:a. Flaming the inoculating instrument prior to and after each inoculation.b. Holding the test tube caps in the hand as illustrated in Figure 1.1 on page 24.c. Cooling the inoculating instrument prior to obtaining the inoculum.d. Flaming the neck of the tubes immediately after uncapping and beforerecappingarrow_forward
- Which of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination testarrow_forwardthirty-six colonies grew on nutrient agar plates when 1.0ml of a 1/10 dilution of a water sample was plated using the standard plate count methodology. How many bacteria were in the original water sample? a.4/ml b.36000/ml c.360/ml d.36/ml e.3600/mlarrow_forwardWhat method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreaderarrow_forward
- Blood agar contains intact sheep red blood cells which are used to determine if a microbe has the ability to lyse these cells. This agar is properly termed A. a selective differential medium B. a complex medium C. a differential medium and a complex medium D. a selective mediumarrow_forwardIn spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.arrow_forwardWhich isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count? A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply. ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms? A. inoculum B. culture medium C. diluent D. microbial culturearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Comprehensive Medical Assisting: Administrative a...NursingISBN:9781305964792Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy CorreaPublisher:Cengage Learning
Basic Clinical Lab Competencies for Respiratory C...NursingISBN:9781285244662Author:WhitePublisher:Cengage
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Basic Clinical Lab Competencies for Respiratory C...
Nursing
ISBN:9781285244662
Author:White
Publisher:Cengage
Infection Prevention and Control; Author: thecityoftoronto;https://www.youtube.com/watch?v=jx9sRYmBW3Q;License: Standard Youtube License