Concepts of Genetics Plus Mastering Genetics with Pearson eText -- Access Card Package (12th Edition) (What's New in Genetics)
12th Edition
ISBN: 9780134811390
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 6, Problem 4PDQ
List all major differences between (a) the F+ × F– and the Hfr × F– bacterial crosses; and (b) the F+, F–, Hfr, and F′ bacteria.
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By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.
Why are the recombinants produced from an Hfr * F- cross rarely, if ever, F+?
An F prime factor:
A) O originates from an Hfr strain
B) O represents an infrequent, abnormal excision of an F factor from a chromosome
C) O contains one or more genes acquired from the chromosome
D) OA, B, and C are all correct
Chapter 6 Solutions
Concepts of Genetics Plus Mastering Genetics with Pearson eText -- Access Card Package (12th Edition) (What's New in Genetics)
Ch. 6 - When the interrupted mating technique was used...Ch. 6 - In a transformation experiment involving a...Ch. 6 - In complementation studies of the rII locus of...Ch. 6 - A 4-month-old infant had been running a moderate...Ch. 6 - Prob. 2CSCh. 6 - Prob. 3CSCh. 6 - Prob. 4CSCh. 6 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 6 - Review the Chapter Concepts list on p. 123. Many...Ch. 6 - With respect to F+ and F bacterial matings, answer...
Ch. 6 - List all major differences between (a) the F+ F...Ch. 6 - Describe the basis for chromosome mapping in the...Ch. 6 - In general, when recombination experiments are...Ch. 6 - Why are the recombinants produced from an Hfr F...Ch. 6 - Describe the origin of F bacteria and merozygotes.Ch. 6 - In a transformation experiment, donor DNA was...Ch. 6 - Describe the role of heteroduplex formation during...Ch. 6 - Explain the observations that led Zinder and...Ch. 6 - Prob. 12PDQCh. 6 - Two theoretical genetic strains of a virus (abc...Ch. 6 - The bacteriophage genome consists of many genes...Ch. 6 - If a single bacteriophage infects one E. coli cell...Ch. 6 - A phage-infected bacterial culture was subjected...Ch. 6 - In recombination studies of the rII locus in phage...Ch. 6 - In an analysis of rII mutants, complementation...Ch. 6 - If further testing of the mutations in Problem 18...Ch. 6 - Using mutants 2 and 3 from Problem 19, following...Ch. 6 - During the analysis of seven rII mutations in...Ch. 6 - In studies of recombination between mutants 1 and...Ch. 6 - Prob. 23ESPCh. 6 - An Hfr strain is used to map three genes in an...Ch. 6 - A plaque assay is performed beginning with 1 mL of...Ch. 6 - In a cotransformation experiment, using various...Ch. 6 - For the experiment in Problem 26, another gene, g,...Ch. 6 - Bacterial conjugation, mediated mainly by...Ch. 6 - A study was conducted in an attempt to determine...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:What information do you know based on the question and your understanding of the topic?arrow_forwardBacteriophage P22 was used in generalised transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the recipient strains of S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the selected for one of the three genetic markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the other two markers. markers. The results are given in the following table: Strain I donor str thrA aceA thrA str aceA+ Strain recipient strs thrA+ aceA thrA str aceA Phenotype selected Str Ace+ Str recombinants selected ThrA ThrA ThrA ThrA Ace Ace Number 60 40 95 5 10 90 str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in threonine biosynthesis. 1) What are the selective media used in these three transduction experiments? to obtain the selected…arrow_forwardTime mapping is performed in a cross involving the genes his, leu, mal, and xyl. The recipient cells were auxotrophic for all four genes. After 25 minutes, mating was interrupted with the following results in recipient cells. Diagram the positions of these genes relative to the origin (O) of the F factor and to one another. (a) 90% were xyl+ (b) 80% were mal+ (c) 20% were his+ (d) none were leu+arrow_forward
- A bacterial strain with genotype his− leu− thr− is transformed with DNA from a strain that is his+ leu+ thr+. A few leu+ thr+ cells and a few his+ thr+ cells are found, but no his+ leu+ cells are observed. Which genes are farthest apart?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Explain how these results are consistent with the idea that thebacterial chromosome is circular?arrow_forwardConsider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forward
- With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it established that physical contact between cells was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis for a bacterium’s being F+?arrow_forwardIn E. coli, four Hfr strains donate the following markers,shown in the order donated:Strain 1: M Z X W CStrain 2: L A N C WStrain 3: A L B R UStrain 4: Z M U R BAll these Hfr strains are derived from the same F+ strain.What is the order of these markers on the circularchromosome of the original F+?arrow_forwardWhen the interrupted mating technique was used with five different strains of Hfr bacteria, the following orders of gene entry and recombination were observed. On the basis of these data, draw a map of the bacterial chromosome. Do the data support the concept of circularity?Hfr Strain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A Barrow_forward
- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:What topic in genetics does this question address?arrow_forwardIn five Hfr strains, each of which was used to build a time-of-transfer map, the genes entered the recipient cells as follows: Strain 1: S L A C T F Strain 2: N P F T C A Strain 3: T F P N U Y Strain 4: S H Y U N P Strain 5: U N P F T C Which of the following represents a correct gene map of these results? N P F T S L A C H U Y S L A C T F P N H Y U C T F P N U Y H S L A T C A L S P N U Y H F U N P C A L S F T H Yarrow_forwardIn E. coli, the genes for histidine (his), arginine (arg), tyrosine (tyr), and valine (val) synthesis are closely linked. A wild-type strain is used as a donor for transducing viruses. Various recipient strains were treated with these viruses. Wild-type recombinant colonies were then counted, with the results shown in the following table. Genotype of recipient Number of wild-type colonies Genotype of recipient Number of wild-type colonies his- arg+ val+ tyr+ 254 his+ arg- val- tyr+ 132 his+ arg+ val- tyr- 36 his+ arg- val+ tyr+ 240 his- arg- val+ tyr+ 28 his- arg+ val- tyr+ 102 his- arg+ val+ tyr- 100 his+ arg- val+ tyr- 2 What is the cotransduction coefficient of histidine and valine? For consistency, standardize on histidine. 0.38 (wrong) 0.537 0.402 None of thesearrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license