Microbiology Fundamentals: A Clinical Approach - Standalone book
Microbiology Fundamentals: A Clinical Approach - Standalone book
2nd Edition
ISBN: 9780078021046
Author: Marjorie Kelly Cowan Professor, Jennifer Bunn
Publisher: McGraw-Hill Education
Question
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Chapter 7, Problem 1CT
Summary Introduction

To describe:

Dramatically the interaction of a holoenzyme and its substrate and general products that can be formed from a reaction.

Concept introduction:

Enzymes are biological catalysts that are proteinaceous in nature. However, there is a special class of enzymes that are made of RNA and can be classified as conjugated or simple.

Expert Solution & Answer
Check Mark

Answer to Problem 1CT

Correct answer: The enzyme-substrate reaction is represented as below:

Microbiology Fundamentals: A Clinical Approach - Standalone book, Chapter 7, Problem 1CT

Fig.1: Enzyme-substrate reaction.

Explanation of Solution

Simple enzymes contain only protein portion while conjugated enzyme refers to holoenzyme which contains apoenzyme and cofactors. Apoenzyme is the actual protein portion while cofactors are either organic (coenzyme) or inorganic elements (metal ions).

For a reaction to takes place, enzyme substrate temporary union occurs at the catalytic or active site. The bond between enzyme and substrate are weak and once the complex has formed, appropriate reaction occurs on the substrate with the help of cofactor/coenzyme. This results in formation and release of products.

Conclusion

Holoenzymes are conjugated enzyme with apoenzyme and cofactors. The reaction between holoenzyme and substrates results in products.

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