Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 7, Problem 3Q
Summary Introduction

To write:

The steps involved in the gram staining procedure. The appearance of the color of gram negative and positive cells in each step in the given table.

Introduction:

Heat fixing:

After the drying of smear at the room temperature on the slide. This slide is gripped with the help of tongs or a clothespin. Then it is passed across the flame of a Bunsen burner and numerous times to heat-kill. It attaches the organism to the slide. Heat fixation usually conserves generally the morphology but not an internal structure.

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List the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.
Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the Gram staining procedure
Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the Gram-staining procedure.
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