Chapter 7.3, Problem 14RQ

What is the purpose of flame sterilizing the inoculating loop or needle before and after usingit? What is meant by ‘streak dilution’ technique? What is the purpose of placing the inoculated agar plate in an inverted position in theincubator?

Differentiate sterilization from disinfection. Differentiate sterilization from disinfection.

Differentiate therapeutic sterilization from direct sterilization What are some of the classifications of sterilization?

What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube

Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5%         Sodium hypochlorite 0.05%         Alcohol 70%         Alcohol 40%         Iodophor (Betadine) 10% solution         Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%

Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing        ________________________________________________ For isolation of colonies        ___________________________________________________

Give at least five different microbial control methods you have on your home as listed on the pre-task activity. Identify its microbial control method (Physical or chemical), Specific component (heat, phenol, etc), Mode of action (denatures protein, disrupt cell membrane, etc), Use (sterilize, disinfect, antiseptic), Level of activity (Sterilant, high, intermediate, low disinfectant)  MICROBIAL CONTROL METHOD SPECIFIC COMPONENT MODE OF ACTION USE LEVEL OF ACTIVITY

Distinguish the difference between a sterile media plate and a contaminated media plate.

Identify the principles and procedures related to disinfection and sterilization.