Chapter 7.3, Problem 5RQ

Describe the Gram staining procedure

Name all the reagents used in Gram-staining and their roles in the staining procedure.

Give atleast two other examples of samples that are best prepared using Smear Preparation Technique.

Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the Gram-staining procedure.

If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?

How is the correct way of incubating culture plate media? Explain why is that so.

in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?

Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing        ________________________________________________ For isolation of colonies        ___________________________________________________

Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation?