Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN: 9781133893943
Author: ESTRIDGE
Publisher: Cengage
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What color wouod gram negative cells appear if the decolorizing step was left out of staining procedure? Why?
Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?
Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
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- What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the gram decolorizer?arrow_forwardWhy must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?arrow_forwardWhy must young cultures be used when doing a Gram stain?arrow_forward
- Which step of the gram stain procedure is most critical?why?arrow_forwardWhy are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?arrow_forwardWhat are the principle and basic concepts of GRAM STAIN? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)arrow_forward
- What is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedarrow_forwardYou mistakenly confused the primary stain and counter stain with crystal violet, how does you misture now appear when viewed with oil immersion?arrow_forwardIn gram staining, could colors other than red be used as a counterstain?arrow_forward
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