Chapter 7.4, Problem 5RQ

If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?

during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.

Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on?   *

Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing        ________________________________________________ For isolation of colonies        ___________________________________________________

Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.

Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.

Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be able to determine if coliforms are present or not.

What is the purpose of flame sterilizing the inoculating loop or needle before and after usingit? What is meant by ‘streak dilution’ technique? What is the purpose of placing the inoculated agar plate in an inverted position in theincubator?

procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slant