Genetic Analysis Format: Kit/package/shrinkwrap
Genetic Analysis Format: Kit/package/shrinkwrap
3rd Edition
ISBN: 9780135194096
Author: Sanders, Mark F.^bowman, John L.
Publisher: Prentice Hall
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Chapter 9, Problem 1P

Some proteins are composed of two or more polypeptides. Suppose the DNA template strand sequence 3'-TACGTAGGCTAACGGAGTAAGCTAACT-5' produces a polypeptide that joins in pairs to form a functional protein.

What is the amino acid sequence of the polypeptideproduced from this sequence?

What term is used to identify a functional protein like this one formed when two identical polypeptides jointogether?

Expert Solution & Answer
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Summary Introduction

To analyze:

Few proteins are composed of two or more polypeptides. Assume the DNA template strand sequence 3’-TACGTAGGCTAACGGAGTAAGCTAACT-5’ yields a polypeptide that joins in sets to form a functional protein.

From this sequence, the amino acid sequence of the polypeptide produced is to be explained.

Name the term used to identify a functional protein which is produced from the joining of two identical polypeptides.

Introduction:

In molecular biology, the central dogma describes the formation of a polypeptide chain from the template DNA strand through the intermediate formation of mRNA.

Transcription    Translation

DNA → mRNA → polypeptide

The DNA contains the information for the given polypeptide in the form of specific sequences of codons. The codon is a triplet of nitrogen bases coding the specific amino acid. There are 64 codons which code 20 different types of amino acids. These amino acids are bounded together by a peptide bond to form polypeptide. The number of polypeptides bounded together form the protein molecule.

Amino acid → polypeptide → Protein molecule

Explanation of Solution

The DNA template will undergo the following process to form the polypeptide chain:

3’ TACGTAGGCTAACGGAGTAAGCTAACT5’ DNA template

↓ Transcription

5’AUGCAUCCGAUUGCCUCAUUCGAUUGA3 mRNA

↓ Translation

N-Met-His-Pro-Ileu-Ala-Ser-Phe-Asp-Stop-C Polypeptide chain

Functional proteins are made up of two or more polypeptide chains which may be same or different to each other. As the two polypeptides chains are identical in nature, they will join together to form a homodimer.

Conclusion

The amino acid sequence of a polypeptide chain is determined by the sequence of codons present on the DNA template strand.

The term “homodimer” is used when two identical polypeptide chains are bounded together.

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Consider the following peptide to answer the questions below: A M A K K E S H I F T A I D E What is the total charge of this peptide at pH = 5? What is the N terminal amino acid (full name)? C terminal amino acid? How many fragments would result if this peptide underwent proteolytic cleavage with V-8 protease and what are these fragments (list them)? Would this peptide be a candidate to form an alpha helix? Why or why not
Remembering that the amino acid side chains projecting from each polypeptide backbone in a β sheet point alternately above and below the plane of the sheet, consider the following protein sequence: Leu-Lys-Val-Asp-Ile-Ser-Leu-Arg- Leu-Lys-Ile-Arg-Phe-Glu. Do you find anything remarkable about the arrangement of the amino acids in this sequence when incorporated into a β sheet? Can you make any predictions as to how the β sheet might be arranged in a protein?
Consider the following in light of the concept of levels of structure (primary, secondary, tertiary, quaternary) as defined for proteins. (a) What level is shown by doublestranded DNA? (b) What level is shown by tRNA? (c) What level is shown by mRNA?

Chapter 9 Solutions

Genetic Analysis Format: Kit/package/shrinkwrap

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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY