Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)
13th Edition
ISBN: 9780134688640
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2R
Differentiate the following terms. Which one is “hit and miss”— that is, does not add a specific gene to a cell?
- a. protoplast fusion
- b. gene gun
- c. microinjection
- d. electroporatio
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Chapter 9 Solutions
Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Answer the two parts of the question. a) Explain what gene therapy involves.b) Discuss how gene therapy can be used to treat/cure or prevent a disease and include in your answer any current challenges associated with its use.arrow_forwarda. You have a test tube containing 5 ml of a solution of bacteriophages, and you would like to estimate the number of bacteriophages in the tube. Assuming the tube actually contains a total of 15 billion bacteriophages, design a serial dilution experiment that would allow you to estimate this number. Ideally, the final plaque-containing plates you count should contain more than 10 and fewer than 1000 plaques.arrow_forwardSection two. Match horizontal gene transfer mechanism with best definition. Answers are used more than once. а. Transduction b. Transformation Conjugation c. 44. requires two live cells, where the recipient receives donor DNA 45. involves a virus 46. usually a plasmid is transferred 47. does not require a virus or another cell 48. "sex" for bacteria 49. occurs because of mistake made in chromos ome packaging by a virus 50. Frederick Griffith coined this term.arrow_forward
- 4) Most embryonic stem cells used for research are: Answer: A) totipotent B) pluripotent C) multipotent D) all of the abovearrow_forwardGive a schematic diagram of how we can Treatment Hemophilia by using gene therapy? Please answer at your own words,please..arrow_forwardSuppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and debris. Describe different commonly used analytical methods to i) separate and purify DNA contents from other cellular contents. ii) amplify a gene of interest if present in the genomic DNA.arrow_forward
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