EBK BIOLOGY
5th Edition
ISBN: 9780100667976
Author: Maier
Publisher: YUZU
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Chapter 9, Problem 3LTB
Summary Introduction
To write:
The process of nuclear transfer in cloning.
Introduction:
The cloning can be defined as the process, in which one cell is multiplied into multiple cells having the same genetic composition. There are different methods used for the cloning of the cells.
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- Describe the various types of vectors used in DNA cloning. Explain the difference between a cloning vector and an expression vector.arrow_forwardDiscuss the Cloning of Chimeric DNA.arrow_forwardList all steps involved in a cloning workflow. Name and link the appropriate enzymes to the steps at which they are used.arrow_forward
- With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forwardDefine cloningarrow_forwardDescribe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.arrow_forward
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