Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 4CT
Summary Introduction
To determine:
The microscopic appearance of the result of the Dorner endospore stain.
Introduction:
When the bacterial cells are in a dormant state, then they form endospores. In dormancy, cells do not undergo
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what are the positive and negative results of the endospore stain procedure?
The primary stain used in the endospore stain is:
A. Carbolfuchsin Stain
B. Methylene Blue
C. Malachite green
D. Safranine
Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
Cite the purpose of each of the following reagents in a differential staining procedure.
Primary stain
Mordant
Decolorizing Agent
Counter stain
What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
Chapter 9 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 9 - What are the Gram reactions of Clostridium and...Ch. 9 - How might a capsule contribute to pathogenicity?...Ch. 9 - Prob. 3QCh. 9 - The flagella stain uses crystal violet, as does...Ch. 9 - How would an endospore stain of Mycobacterium...Ch. 9 - What type of culture medium would increase the...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Endospores appear as colorless areas in a simple...
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- What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.arrow_forwardWhy is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?arrow_forwardAdditional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?arrow_forward
- Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a acid alcohol b crystal violet c carbolfuschin d malachite green e safranin f iodine g alcohol h methylene bluearrow_forwardBacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorizationarrow_forwardName the reagents used in Gram-staining and their roles in the staining procedurearrow_forward
- What color wouod gram negative cells appear if the decolorizing step was left out of staining procedure? Why?arrow_forwardIn preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?arrow_forwardThe acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?arrow_forward
- What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forwardIs heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?arrow_forwardWhat is the difference between Giemsa and Wright stain?arrow_forward
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