DNA ligase

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  • Dna Ligase Essay

    1320 Words  | 6 Pages

    three major types of DNA ligases are reported: DNA ligase I (hligI), DNA ligase III (hligIII) and DNA ligase IV (hligIV). hligI plays an important role in DNA replication by joining Okazaki fragments on the lagging strand of DNA. Apart from this it also plays important roles in DNA damage repair pathways, single strand breaks are repaired by nucleotide excision repair (NER) and base excision repair (BER) pathways. Results of preclinical studies support that Human DNA ligases are an attractive target

  • Liddle's Syndrome

    1727 Words  | 7 Pages

    mRNA of the PY motif for ENaC(ref). This results in either a truncated protein or elimination of some amino acids from the PY motif, which interfere with the binding ability of Nedd4 ligase and inhibit Nedd4 ligase binding, respectively. Overall, this either decreases or eliminates the possibility of Nedd4-2 ligases’ ability to tag and degrade the ENaC. With the inability to control ENaC surface expression at the kidney, its reabsorption of sodium will be markedly increased due to

  • Taking a Look at DNA Cloning

    1260 Words  | 5 Pages

    DNA cloning is the process of creating a multitude of copies of isolated DNA fragments; DNA cloning can be carried out via in vitro or in vivo methods. One can clone a specific DNA sequence or entire gene fragments. There are a multitude of procedures to carry out DNA cloning, but the major steps are the same for all types. To begin the process, one must isolate a DNA fragment from the chromosomal DNA. This is done by using a restriction enzyme. One could also use gel electrophoresis and polymerase

  • Dna Synthesis Case Study

    403 Words  | 2 Pages

    achieve accuracy of DNA replication after the unwinding of DNA, the DNA must be synthesized. DNA synthesis, in the case, the two strands are disconnected and then turned into single-stranded DNA. This is called replication fork. The replication fork acts as a model for DNA synthesis. However, the site of impaired or mutated DNA can cause a lower rate pf success for DNA synthesis. Impaired DNA can also lead to unprocessed and unligated okazaki fragments. Okazaki frgaments are small DNA fragments that are

  • Ampicillin In Bacteriophage Experiment

    577 Words  | 3 Pages

    ampicillin in the agar solution was working. Reactions 1-3 were set up to locate the optimum ratio of bacteriophage insert DNA to create the most

  • Dna Ligation Lab Report

    682 Words  | 3 Pages

    An important reaction that needed to be performed before introducing foreign DNA into E. coli is the ligation reaction. DNA ligation is a process that joins DNA fragments together by creating a phosphodiester bond between the 3’ OH of the nucleotide and the 5’ phosphate of another. An essential enzyme that ligates a DNA fragment and a vector together is the T4 DNA ligase, which originates from T4 bacteriophage. This enzyme will ligate cohesive ends produced by restriction enzymes. T4 will also ligate

  • The Applications Of Genetic Engineering

    1897 Words  | 8 Pages

    together to repair the DNA strand by joining together. There are two main types of DNA ligase, the first is found only in prokaryotic cells. The second is found in eukaryotic cells . Furthermore, mammals have four subtypes of ligases that vary in their function; DNA ligase III, this contains a DNA repair protein, called XRCC1, that helps to repair the DNA strand that occurs during nucleotide repair. Eukaryotic DNA ligases are larger than the prokaryotic cells. Therefore DNA ligase has a very important

  • Klhl3 Gene Essay

    3060 Words  | 13 Pages

    the KLHL3 protein has a N-terminal BTB domain, a C-terminal that has Ketch-like repeats, and a BACK domain, forming a conformation that is a bladed beta propeller structure (1). The protein produced by this gene is part of a complex (E3 ubiquitin ligase), which functions to indicate damaged and additional proteins by labelling these with ubiquitin, which is the tag later recognized by proteasomes for breakdown (4). This labelling is possible with the

  • Biology Tools

    1045 Words  | 5 Pages

    enzymes are DNA-cutting enzymes found in bacteria (and harvested from them for use). Because they cut within the molecule, they are often called restriction endonucleases. In bacteria the restriction enzymes naturally provide immunity to the bacteria against bacteriphage by cutting viral DNA is that introduced into the cell (Fig. 2 Page 280). After being cut, the viral DNA becomes inactive and thus the phage cannot attack the bacterial cell. A restriction enzyme recognizes and cuts DNA only at a

  • Discover Therapeutic Aptamers For Vegf165 And Egfr

    2025 Words  | 9 Pages

    Discover therapeutic aptamers for VEGF165 and EGFR by high-fidelity pentamer condon set Dehui Kong, Department of Chemistry, University of Georgia Introduction Aptamers are ssDNA or RNA oligonucleotides with very high affinity for their target. They bind to their target with high selectivity and specificity because of their well-defined tertiary structures. Researchers speculate that aptamers have the potential to replace antibodies as high-affinity reagents in medical diagnostics and therapy. SELEX