Gram-positive bacteria

the “Gram- Positive Cocci in clusters” flow chart which comes from the class lab manual. After performing a hand full of tests, I came to a conclusion that organism 51 that I had chosen was St. mitis. I noticed that this organism was streptococcus but had both an individual and a chain like arrangement. The noted streak plate colony growth was round in shape, slightly elevated, smooth in texture and translucent or partially transparent. The initial test that I had performed was the Gram staining

| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing

other etiologies that lead to the development of further organ injury, ultimately culminating in multiple organ dysfunction syndromes. The word “bacteraemia” is used when bacteria are isolated from the blood whether clinical symptoms are present or not while the words “sepsis” or “septicaemia” is used for the isolation of bacteria in blood in combination with clinical symptoms and signs of infections. Neonatal sepsis, also named “Sepsis neonatorum”, represents to a group of physical and laboratory

Results and discussion Esterification extent: The color reaction using hydroxylamine hydrochloride was used with modification to quantify the extent of esterification of proteins and the results showed that the highest level of esterification was recorded for esterified cowpea protein isolate (82%) followed by esterified common bean protein isolate (79%). Functional properties of native and modified proteins: PH-solubility profile: The pH-solubility curves of native and esterified cowpea protein

to gram stain, and hopefully separate them. After following the procedures for gram staining, and observing my slide underneath the microscope, it was evident that I had two microbes on my slide. One of the microbes was gram positive with a coccus shape, while the other was gram negative with a small rode looking shape to it (Picture A). The next step was to be able to separate them from each other using selective media. For the gram positive unknown, I used a MSA plate, which prevented gram negative

incubated for 24-48 hours. The morphological test performed on the unknown #462 were all consistent with the expected results for this bacterial species such cell shape, motility, and gram stain. The bacterium was gram positive was confirmed by growth on PEA plate, inhibition of growth on EMB plate, and through gram staining. The optimal growth temperature was at 37˚C which corresponds to the optimal temperature mesophilic bacterium as stated in StrainInfo2. Cultural characteristics of unknown #462

After several biochemical tests, Unknown Bacteria #30 was identified as Staphylococcus aureus. After growing the bacteria on Nutrient Agar to ensure a pure sample, it was Gram stained to determine morphology and arrangement. It was observed to be a Gram positive staphylococci. Then, the bacteria was inoculated onto a Mannitol Salt Agar plate. After incubation, it was observed to have bacterial growth and the agar was yellow in color. According to the lab manual (2), MSA contains 7.5% NaCl and

mercury as pollutant into water bodies has arise the level of mercury from 20 to 100 times or even more from the permissible limit of it. The contamination has affected the marine population at large but few of bacteria have found way out to tolerate such high concentration of mercury. Such bacteria have been discovered way back in 1960’s by Moore, where he reported to found an operon called as mercury operon or mer operon which is found to be integrated on plasmid, transposons, etc., which harbors the

After a few days of growing, the presence of two unknown bacteria had a clear visible of separation. Following the isolation streak technique, the gram-staining experiment was performed. The two bacteria were determined: one was Gram-negative bacteria and the other was Gram-positive bacteria. With the new batch of bacteria, a series of biochemical tests were performed. All the biochemical tests performed to identify two unknown bacteria were Phenol Red Broths, nitrate reduction,

semi-synthetic penicillin), to curb the resistance problem resulted in the evolution of another resistant strain known as methicillin-resistant Staphylococcus aureus (MRSA).4 Figure 1: Some β lactam antibiotics Resistance to β lactams is easy for bacteria as all β lactams shared the same mode of action, which is the inhibition of bacterial cell wall synthesis by forming stable covalent adducts with the active site serine residues of penicillin binding proteins (PBPs). The PBPS are often divided into