Gram-positive bacteria

Gram Stain In 1884, a Danish pathologist, Christian Gram, discovered a method of staining bacteria with pararosaniline dyes. By using two different dyes, he discovered that bacteria fall into two different groups. The first group, gram positive, retains the primary color, crystal violet. The second group, gram negative, when washed in a decolorizing solution, becomes colorless and takes on the color of the counterstain. The reaction of the Gram-stain in a bacteria is determined by the biochemical

12th December, 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria, which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of identification

that falls, and the bacteria that get you sick may both be small, but when you allow them to accumulate, you can see characteristics of them. While snow is made from frozen water, with flakes differentiating in the structure of them, bacteria is like that. Certain bacteria families may have similar genomes, they do have slight differences that make up what they are. One way to tell the difference is to see if the bacteria is gram positive or gram negative. Gram-Positive bacteria contains peptidoglycan

Microbial Unknowns Objective: In the past couple of weeks, our class received a single broth that contained two species of bacterium. One bacterium was gram positive and one bacterium was gram negative. We were required to use our techniques and knowledge we learned in class to separate and identify the two species we were given in our broths. Methodology: Day One: On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of

The objective of this experiment was to identify two unknown bacteria from a mixed culture. Which was done by using the aseptic technique which was very important to avoid any contamination and keeping the workspace clean while culturing bacteria for different tests. To start, I chose a tube which had a solution of mixed culture. I used the flame to sterilize the inoculating loop and dipped it into the tube and streaked for isolation on 2 TSA plates and placed them in an incubator at 37 for 24 hours

class of bacteria present in the gastrointestinal system of humans and other mammals. It can cause urinary tract infections and endocarditis (most commonly) and can be life-threatening to humans. In this study, an unknown bacteria was assigned and had to be identified through biochemical tests. After preparing a working plate, a Gram staining test, a catalase test, and a RBC hemolysis test were done for the identification. The Gram staining resulted in the unknown bacteria being a Gram-positive coccus

Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential

identify the bacteria. Initially a broth of an unknown was given containing both positive and negative unknown bacteria. It is important to observe aseptic technique to successfully and accurately identify the unknown bacteria that was given. Aseptic technique is used to avoid contamination. This could vary from contamination from the air to contamination with the materials that are being used for the experiment. If contamination occurs, then the unknown could produce a false result. Bacteria is usually

identify bacteria species based on their differences in their biochemical activities of different bacteria. To differentiate one bacteria from another, each species of bacteria has a well-defined set of metabolic activities. Bacteria can be differentiated in many ways. They can be based on structural differences or metabolic differences. Structurally, the Gram stain is the most fundamental technique in identifying bacterial species and was developed by Christian Gram in 1884. Gram negative bacteria can

strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed. Introduction Gram staining is a technique used to determine if the bacteria is Gram positive