Gram-positive bacteria

semi-synthetic penicillin), to curb the resistance problem resulted in the evolution of another resistant strain known as methicillin-resistant Staphylococcus aureus (MRSA).4 Figure 1: Some β-lactam antibiotics Resistance to β-lactams is easy for bacteria as all β-lactams shared the same mode of action, which is the inhibition of bacterial cell wall synthesis by forming stable covalent adducts with the active site serine residues of penicillin binding proteins (PBPs). The PBPS are often divided into

Organismal Reports Micrococcus luteus Micrococcus luteus was first isolated and discovered in 1929 by Alexander Fleming. Is a gram positive bacteria that is commonly found on human skin, water, soil and dust. Since it is an obligate aerobe and thrives in the presence of oxygen, it is also found in the human respiratory tract. It takes the shape of cocci and is often clumped irregularly, in tetrads, or groups of four. It is also non-motile and does not form endospores. Although it has a specific optimal

Staphylococcus aureus embraces many characteristics which are derived by a variety of test to identify if someone has this certain bacteria. This specific species is recognized by the anaerobic fermentation, beta hemolytic halo, coagulase positive testing, the Kirby-Bauer disk diffusion test resistance, clumps of gram positive cocci that is identified through the microscope, and the a positive catalase test (Kiser 67-87). First in the series of test was the mannitol salt agar MSA plates, which contains a high

our discovery for our unknown, we were instructed by Professor Do to carry out a streak technique on a MacConkey’s Plate for general growth. We also had to do a streak technique onto a Nutrient Agar Plate in order to separate the Gram-Positive microbe from the desired Gram-Negative microbe that we were trying to identify. Before the streak technique was performed, the sample was “vortex” to mix solution, then a loop was sterilized with a flame. The loop was then used to aseptically inoculate a sample

evaluated using DPPH and ABTS assays and the Folin-Ciocalteu reagent method to determine total phenolic content of the MEOG.The antimicrobial effect was determined by agar diffusion and broth dilution method against different ATCC strains of pathogenic bacteria. Zones of inhibition, minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) were determined. The results indicate that the antioxidant potential and antimicrobial activity of MEOG is mainly due to its total phenolic content

The implication of the contaminants may be due to human intervention in gram positive bacteria, and maybe because of environmental process control including raw materials and water systems for gram negative bacteria (Jimenez, 2004). Among Gram-negative bacteria, rod-shaped organism are the most common bacteria found in non-sterile pharmaceuticals, since can be acquired environmental (Jimenez, 2004). A study on microbiological quality of lotions

Gram Negative Salmonella Typhimurium (#16) Lab Report Heather A. Rogers Nov. 9 2016 Biol 2460 University of Texas at Arlington  Abstract This lab experiment was done for the purpose of learning how to determine a gram negative bacterium based on multiple tests learned throughout the semester. My gram negative unknown bacterium given to me was Salmonella typhimurium based off of the following tests; Triple Sugar Iron Agar (TSIA), Sulfate Indole Motility (SIM), Methyl Red (MR), Voges-Proskaur (VP)

of this investigation was to determine whether bacteria commonly isolated as a pair from contact lens cases could coaggregate or cohere. The organisms were used in this study was isolated from the contact lens cases of asymptomatic daily wearers and stored in culture stock. The primary outcome of this study demonstrated that coaggregation and cohesion occurred between Acinetobacter spp, Staphylococcus spp and Micrococcus spp. Two Gram positive bacteria, Staphylococcus spp and Micrococcus spp also showed

The first test done for Klebsiella pneumoniae (the gram stain) indicated that this bacteria was rod shaped and therefore, using the dichotomous key the family of bacteria that were ruled out consisted of the genera Neisseria and Rodospirillum. The next tests that were vital to the identification process were the oxygen requirements and glucose fermentation tests. The test results gave us the confirmed family of our species which was Enterobacteriaceae. The negative oxidase test performed confirmed

It is estimated that 200,000 to 400,000 hospitalized patients with central lines develop bloodstream infections annually in the United States. The prevalence of central line-associated blood stream infections (CLABSIs) have increased, and significantly caused morbidity and mortality in patients. They are a leading cause of preventable health care-associated infections (HAIs) and have resulted in longer hospital stays and higher hospital costs. In order to combat this epidemic, the Centers for