Gram staining

the solid medium surface. I placed a loopful of the culture on the agar surface using the streak plate technique and gave it some time to grow. By the following week, I had heavy growth on my agar surface (image on the right side). Differential staining requires the use of at least four chemical reagents that are applied in a specific order to a heat fixed smear. The primary stain is used to impart color to all cells leads into the second stain; the mordant used to make the color of the primary

two bacteria species; one gram-positive and one gram-negative. A dichotomous key was used in order to determine what tests needed to be conducted to define the particular bacteria. It is highly important for the tests to be followed as specified in the manual; otherwise, the bacteria could be falsely identified. Controls must be included in the testing to make sure there is something to compare the unknown results to. The tests conducted in this experiment included, gram stain, SIM, citrate, nitrate

This report discusses the procedures and equipment needed to determine a bacterium knowing and having nothing but the sample bacteria given by the instructor. The importance of this lab is to test your ability to think clearly about what each test will tell you about your unknown organism and selecting tests that will save time and give you the most information. The objective of this lab is to determine what species level and genus level your bacterium is categorized as. The first step taken to

Kelsey Nolte Microbiology Otero Junior College Mr. McClure Fall Semester 2014 Unknown Report Introduction The definition of Microbiology is the branch of science that deals with microorganisms. Microorganisms are very small organisms that require the use of a micro scope to visualize them, which are bacterium, viruses, fungi (the most common) and others which are not so common such as parasites. Microorganisms are an essential element to the field of microbiology because it allows researchers in

Table 3 shows Gram stain results that indicated C. Freundii as a gram negative bacterium in rod shapes scattered in singles and some in pairs. Each gram stain produced the same results. The Bartholomew and Mittwer method of endospore staining indicated that C. Freundii tested negative for endospore formation. Table 4 shows the biochemical test results of the unknown and the official

the unknown organism tested. It is organized by phenotypic characteristics of organisms and conducts a systematic way of identifying the other unknowns. In the lab students were given a tube labeled with a number. Instructions were given to conduct a Gram stain to begin the search followed by the use of a Dichotomous Key and photos as resources to carry out the search. Instructions read to isolate and identify the unknown bacterium with both differential and selective tests to positively identify the

Introduction A microbe is described in the dictionary as “a microorganism, especially a bacterium causing disease or fermentation.” The thought of these bacteria make some people shudder; the thoughts of unseen enemies crawling on surfaces and primary areas of life. To prevent the spread an overgrowth of the organisms, we as a race have invented and developed many innovations. Although these fears are justified, could it be that these microbes we fear so much, exist in greater numbers within us

Introduction: Having a hands-on academic experience of the different techniques for instance aseptic technique and procedures like gram staining is important characteristics for understanding the concept of learning to identify a type of bacteria. During this lab report each student would be using a variety of lab techniques and process/procedures from the lab course taught from the professor and learned from students this semester. To include, by performing the multiple test with three types of

Purpose: The purpose of a Gram Stain procedure is to distinguish bacteria into two fundamental groups of cells. There are the Gram positive cells and the Gram negative cells. By staining the cells either violet or red, we can see the two types and their arrangements on the smear. Theory and Background: 1. After swabbing some microbes from my cheek, I spread the bacterial smear on the slide. In order to make them stick to the slide, I hold it near a heat source so they can attach. Then I flood

was done. A Gram Stain was done and the sample was gram positive. A streak plate revealed that the microbe was circular, convex, yellow, buttery and opaque. The next test was the oxidase test. The microbe was oxidase negative. The next test performed was the catalase test. The microbe was catalase positive. The last test performed was a MSA streak plate. The MSA plate turned yellow. This was the final indicator that the unknown microbe 1D was Staphylococcus aureus. Procedure 1 Gram Stain: Purpose: