Immunofluorescence

Primary Article Assignment Microbiology ICP#1 (Viruses) Name: Katie Slivko ____________________________________________________________________ 1) Anatomy of Paper a) 19 authors (Rodrigo Delvecchio, Luiza M. Higga, Ana Luiza Valadao, Fabio L. Monteiro, Paula Pezzuto, and Patricia P. Garcez) ect. b) They all worked at the Institute of Biology at the Federal University of Rio de Janerio. In addition, it seem that they all had experience with infectious disease. Most of the authors worked in genetics

Cell cycle analysis. PC3 (8×104  cells/well) and LNCaP (3×105  cells/well) cells were seeded in 6-well plates in 1.5  mL of complete growth media. Cells were treated with 2.5 μM of free sorafenib or an equivalent sorafenib concentration of SMA-Sor, 3 μM of free nilotinib or an equivalent nilotinib concentration of SMA-Nilo, DMSO or SMA for 48  h. Cell cycle distribution was assessed using propidium iodide staining, as previously described {Somers-Edgar, 2011 #82}. Samples were analysed using a FACScalibur

In order to test this hypothesis, the team determined that the mor1 locus in a mutant A. thaliana plant caused temperature sensitive mutations in the development of microtubules when the temperature was increased from 21 C to 29 C. They used immunofluorescence microscopy, a technique that stains cell structures with a fluorescent dye, to visualize the actual microtubules of both wild type and mutant A. thaliana at different temperatures. The mutant A thaliana, were determined to contain two mutant

Lyme disease begins with a bite from an infected tick. It is the most common tick-borne disease within the United States (Wadsworth Center, n.d.). The causative agent of Lyme disease is Borrelia burgdorferi. This pathogen is a spirochete with endoflagella, an outer membrane with LPS-like substance, an inner membrane and a periplasmic space which contains a layer of peptidoglycan (Tilly, Rosa, Stewart, 2009). As stated before, it is transmitted via a bite from an infected tick. There are two main

By Dr Liji Thomas, MD Vasculitis is an inflammatory disorder which affects the wall of blood vessels in the skin. It may be classified based on the type of vessel involved, as small vessel, medium-vessel and large vessel vasculitis. The inflamed vessel wall undergoes destruction by the inflammatory infiltrate, resulting in any of the following: hemorrhage from the disrupted blood vessel aneurysm of the weakened wall hyperplasia of the intimal and medial layers leading to vessel narrowing ischemia

Cystic fibrosis (CF) is a life-threatening genetic disease, explicitly an autosomal recessive genetic disorder, which primarily affects the lungs and digestive system. An estimated 30,000 children and adults in the United States have CF (Song, Chiu, & Yoon, 2012). According to the Cystic Fibrosis Foundation, “In the 1950s, few children with CF lived to attend elementary school”("Cystic Fibrosis Foundation," 2014, p. 1). It is important to recognize the progress that has been made on diagnosing

Introduction Stem cells are homogeneous cells found in the human body which have potential to change into, or differentiate into a range of specific types of cells (Stoppler, 1996). The refurbishment and protection of many adult tissues are safeguarded by stem cells (SCs) which exist in the top of the cellular sorting section of these tissues (Beck, 2013). Practical assessments, such as transplantation, in vitro clonogenic assesses (technique used to study efficacy of exact agents in terms of the

Allan-Herndon-Dudley syndrome (AHDS) is a rare disorder of brain development that causes severe intellectual disabilities and problems with locomotion. AHDS occurs exclusively in males disrupting development from before birth being a psychomotor retardation characterized by neurological impairment and abnormal thyroid hormone (TH) levels. Past research has shown that mutations in the TH transporter monocarboxylate transporter 8 (MCT8) are associated with AHDS with MCT8 knockout mice exhibiting impaired

Fig. 1. Construction of infectious cDNA clones. (A) A full-length infectious cDNA clone of the PRRSV HuN4 strain was inserted into the pBeloBAC11 plasmid as described in Materials and methods. (B) Two unique restriction sites (AsiS I and Swa I) were introduced into the HuN4 genome. (C) The secreted NanoLuc™ (secNluc) gene driven by a synthetic transcription regulation sequence 6 (TRS6) was cloned into the HuN4 genome. Fig. 2. Characteristics of the recombinant HuN4-secNluc virus. (A) Growth characteristics

Fluorescence-activated cell sorting (FACS) arose from the need to separate and purify specific cells of different organisms or parts of organisms (5). The FACS was created in the late 1960s by Bonner, Sweet, Hulett, Herzenburg, and others and is a special type of flow cytometry(5). The commercial use of the machines did not happen until the early 1970s. The use today mostly involves staining, analyzing and sorting individual cells from small human samples (5). These methods are mainly applied to