Immunofluorescence

Several approaches can be envisaged to address the function of a gene. The techniques of molecular biology and biochemistry allowing for example to localize the expression of a gene or its product (Northern, western, in situ hybridization, immunofluorescence, etc.), to determine the structure of the protein (NMR, crystallography ...) or to find partners proteins (double hybrid, immunoprecipitation ...) give important information on the function of a gene (V Ecochard -2011). This experiment

Jillian Lowery, a 29 year old patient, came in with primary complaints consisting of fatigue, exhaustion, shortness of breath, some nausea, fever, anxiety, light sensitivity and headaches. After receiving Jillian’s history of present illness and family history, it was discovered that her mother, 60, is currently taking Zoloft for depression and her father, 64, was diagnosed with colorectal cancer four years ago and is currently cancer free. Jillian is allergic to Ampicillin and does not take any

Mesenchymal stem cells (MSC) are fibroblast-like structural cells that are found in bone marrow, skeletal muscle, and other connective tissues. MSC derived from bone marrow (BM) have been shown to be self renewing and are capable of differentiation into a variety of stromal cell types including bone forming osteoblasts, fat storing adipocytes, and neurons. Another trait exhibited by BM-MSC is their supportive role in hematopoiesis (blood genesis). This behavior has been utilized to enhance efficacy

immunostaining methods began when Marrack produced reagents against typhus and cholera microorganisms, using a red stain conjugated to benzidin tetraedro (1) However, Professor Albert H. Coons from Harvard School of Medicine first introduces immunofluorescence. Coons initial attempts to label antibodies were unsuccessful as the labels were not visible enough under the microscope. In the early nineteen forties Coons, Creech, Jones and Berliner succeeded in tagging antibodies (2). These antibodies were

If you wish to update or expand upon your activities, you may provide additional information below. [/500] I have recently finished my summer internship at the University of Maryland School of Medicine under the UM Scholars program. My work was very productive and my poster presentation was very well received. Please describe your most recent research project, its importance and how it captured your interest. [492/500] Just this summer, I completed an internship in Dr. Ricardo Feldman’s Lab in the

Cell cycle progression is orchestrated by a complex convergence of many signaling pathways. The decision to activate particular pathways while inhibiting others is mediated by a select group of gatekeeping proteins. At the molecular level, one particular interesting area of research is identifying particular proteins that function as crossroads of t Diverse pathological conditions, such as cancer and atherosclerosis, share a common molecular basis: deregulation of cell cycle progression . In

“No man’s knowledge can go beyond his experience”, this statement by John Locke captures the essence of why I am interested in taking the Chromatin, Epigenetics, and Gene Expression course. I have always had a lifelong thirst for knowledge. As a child I was always asking questions about why or how things worked. I would always tinker with things, taking them apart and reassembling them. I questioned pretty much everything and longed to be able to do experiments and find solutions to unanswered questions

Summary of: Isolation and Characterization of Human Umbilical Cord Mesenchymal Stem Cells with Hematopoiesis-supportive Function and Other Potentials (Lu et al. 2006) Mesenchymal stem cells (MSC) are fibroblast-like structural cells that are found in bone marrow, skeletal muscle, and other connective tissues. MSC derived from bone marrow (BM) have been shown to be self renewing and are capable of differentiation into a variety of stromal cell types including bone forming osteoblasts, fat storing

First off Vivisection is the practice that uses living animals for operations for the purpose of experimentation or scientific research. In shorter words vivisection is a type of animal testing. Aristotle used animal testing in 384-322 BC(Hajar 1). This just shows animal testing has been going on for decades. Vivisection is viewed as a harmful abusive practice to animals. If their was a way to demolish vivisection in the future there would be other alternatives that would replace it. Scientists

PATHOGENESIS Current evidence suggests that mucous membrane pemphigoid develops as a consequence of the loss of immunologic tolerance to structural proteins in epidermal basement membrane. This loss of tolerance culminates in the development of circulating autoantibodies that bind epidermal basement membrane, elicit inflammation, and weaken adhesion of the overlying epidermis. As noted earlier, a variety of different autoantigens are recognized by circulating autoantibodies from patients with mucous