Single domain antibody

Monoclonal antibodies have been previously studied and generated against acute myeloid leukemia cells including anti-CD47 mAbs30, anti-CD33 mAbs31, and anti-CD13 mAbs32. These previous mAbs can elicit an immune system or carry toxins to kill the cancer cell itself. In addition, a procedure in which mice were immunized with the mammalian cells expressing the native proteins on the surface to create mAbs has been previously performed with P. falciparum33. To improve the therapeutic efficacy of mAbs

HIV Broadly Neutralizing Antibodies Introduction: In the late 20th century a new type of virus called Human Immunodeficiency Virus (HIV) was introduced into human beings. There are two types of HIV causing AIDS, HIV-1 and HIV-2. Independent cross species transmission of SIVcpz from chimpanzee and SIVsm from sooty mangabeys resulted in HIV-1 group (M, N and O) and HIV-2 group (A-H) (Keele et al., 2006, Damond et al., 2004). It is notable that the SIV cannot be readily transmitted into humans. Only

polyomaviruses at the early genomic region level, but has a poor homology of sequence with the genomic region Late (165). These multifunctional proteins are coded by five different transcripts that are formed in followed by alternative splicing from a single precursor of mRNA. They play precise roles in regulating the virus cycle and in cell transformation. 1.2.3.1 The large T antigen T-antigen is a protein of 688 amino acids and regulates the transcription of the transcription from early to late proteins

proliferate uncontrollably due to over-expression of the protein HER2 (Human Epidermal Growth Factor Receptor 2); a receptor embedded within the membrane of cells, allowing for the transfer of signals outside to inside the cell. Trastuzumab is a monoclonal antibody administered through intravenous infusion, to be taken on its own or in combination with one or more chemotherapy regimens. It reduces risk of the cancer reoccurring or spreading by inhibiting the effects of HER2, and enhancing the body’s immune

thought to be the barrier of monoclonal antibodies distribution to tumor cells. Fujimori et al. constructed a model which demonstrated increased in antibody affinity and concentration could delayed filtration of the molecule into cells.56, 58 Other studies conduct to support Fujimori’s experiment, which also discover saturation of the binding site barrier to play role in monoclonal antibody to intra-tumor distribution59. • Mechanisms of action: Monoclonal antibodies have several mechanisms of action by

and both upper and lower airway epithelial cells are infected at a similar frequency [7]. Non-enveloped rhinovirus belonging to the Picornaviridae family is spherical in shape, with a diameter ranging from 28 nm to 30 nm. The viral genome contains a single open reading

Purpose: Short chain variable fragments (scFv) have better pharmacokinetic properties and higher yields with lower costs compared to monoclonal antibodies. Chemokine C-C motif receptor type 2 (CCR2) is a chemokine receptor which plays an important role in some inflammatory diseases, infections, and metastasis of cancer. By targeting specific epitope on CCR2 with scfv, can prevent migration of tumor associated macrophages (TAMs) to the tumor mass. We propose that a scFv against CCR2 would compete

Zagazig University Faculty of Medicine Clinical Pathology Department Association of STAT4 Gene Single-nucleotide Polymorphism with Systemic Lupus Erythematosus Thesis Submitted for partial fulfillment of M.D. degree in clinical pathology By Marwa Abd El-Monem Mohamed Ateya MB.B.CH. Ass .lecturer of Clinical Pathology Faculty of Medicine Zagazig University Prof. Dr. Lamiaa Abd Al-wahab Mohammad Professor of Clinical Pathology Faculty of Medicine Zagazig University Prof. Dr. Asmaa

proprotein convertase subtilisin/kexin type 9 (PCSK9) (2). PCSK9 binds to the epidermal growth factor-like domain of LDL receptors on the surface of hepatocyte and targets the receptor for lysosomal degradation (2) In the absence of PCSK9, the LDL receptor will be recycled at the plasma membrane increasing the ability of

In this experiment, the reaction that occurs because of ligand binding to YFP, a dopamine transporter was observed. This experiment required sterility practice, immunofluorescence staining, and colocalization. To accurately conduct immunofluorescence staining, a thorough understanding of proper sterile techniques is needed. Sterility refers to cell culturing experiments without any contamination, therefore all of the experiment was performed under the hood. No discoloration signified no contamination