Transfection

Matrix mettaloproteinase-3 as a potential biomarker for human metastatic osteosarcoma or Elevated expression of MMP-3 in human osteosarcoma and associated with tumor metastasis Abstract: Matrix metalloproteinase-3 (MMP-3, is one of several matrix metalloproteinases (MMPs) family that has been observed in several malignant tumors, including breast, colon, cervical and lung cancers, where its expression correlates with the invasion and metastasis of these tumors. However, the roles of MMP-3 in osteosarcoma

IRF-1/7 Function: IRF-1 has antiviral, immunomodulatory, apoptotic, and immune cell differentiation effects. Experiments in IRF-1 knockout mice have demonstrated that IRF-1 is a key regulator of macrophage function, NK cell response, Th1/Th2 differentiation, and DC differentiation (94-97). IRF-1 is involved in the regulation of many genes during inflammation, immune responses, and cell proliferation. IRF-1 interacts with both IRF-2 and IRF-8 as an antagonist. IRF-2 is an antagonist that represses

condensation ability with the negatively charged plasmid DNA (pDNA) to form copolymer/ pDNA polyplexes, which is an important process to prevent pDNA degradation by nucleases also PDMAEMA-PHB-PDMAEMA/DNA polyplexes displayed significantly better gene transfection when using with luciferase/plasmid in Cos7 and HEK293

function. To perform this experiment they began with a RNA interference and from this generated small hairpin RNAs. These were directed against the rodent Neuroliginds isoforms which were NL-1, NL-2, and NL-3. They then tested their efficiency by co-transfection with Neuroliginds expression vectors. After knocking down the expression of Neuroliginds they were able to create an isoform specific. This resulted in the suppression of excitatory synapses. The next thing they did was a triple knock down of

The RNA-guided CRISPR-Cas9 system has revolutionized genetic engineering by allowing targeted genomic modification through the simple design of a 20 base pair guiding sequences. This groundbreaking technology utilizes a short guide RNA (sgRNA) to direct the Cas9 nuclease to a specific genomic locus through complementary base pairing. The guide RNA or sgRNA is responsible for the specificity of the CRISPR-Cas9 system, and several consideration need to be taken during its design process. In bacteria

to astrocytes and will code for the neurotrophic factor, Neurotrophin-3 (NT-3). Positively charged polyethylenimine (PEI) will be complexed with negatively charged Ψ-mRNA in order to preserve the genetic material’s bioactivity as well as enable transfection of cells.8 I will fabricate this scaffold by electrospinning PEI/Ψ-mRNA polyplexes into aligned poly(lactide-co-ε-caprolactone) (PLCL) fibers that will provide sustained, localized release of the polyplexes and promote directional regeneration

Introduction Osteogenesis Imperfecta is a heterozygous disease that results in increased susceptibility to fractures for an affected individual. This disease is categorized into seven different types based on clinical phenotype. Approximately 90% of Osteogenesis Imperfecta is caused by a dominant inherited mutation in type 1 collagen protein. While 5-10% is caused by a recessive inherited mutation in genes regulating osterobastogenesis, and collagen assembly and processing. Type’s I-IV is associated

Tutorial Assignment 1 1. The title of the article is “ A Functional SNP in BNC2 Is Associated with Adolescent Idiopathic Scoliosis” Journal Reference: Ogura Y, Kou I, Miura S, et al. A functional SNP in BNC2 is associated with adolescent idiopathic scoliosis. The American Journal of Human Genetics. 2015; 97(2): 337-342. doi: 10.1016/j.ajhg.2015.06.012 2. The authors studied this subject as it relates to a very prevalent medical diagnosis around the world, Adolescent idiopathic Scoliosis (AIS)

2.1. Gene Therapy Gene therapy involves the introduction of genetic material into cells in order to treat or prevent disease. Classical gene therapy has been described as “using DNA as a drug”, in which DNA carrying genes is transferred into cells by artificial means. After decades of research, this approach has now been successfully used to treat a number of conditions in humans. This section presents the historical background to the development of gene therapy. Genes as theoretical units of inheritance

Delivery of CRISPR-Cas Components: CRISPR systems are generally delivered in the cell using normal methods of transfection such as microinjection, gene gun, electroporation, sonoporation, and using viruses such as adenoviruses and lentiviruses. In cultured mammalian cells, researchers have used electroporation, nucleofection, and Lipofectamine mediated transfection methods to deliver vectors expressing the gene for gRNAs and Cas9 endonuclease. In cultured human and mouse cells, Lentiviral vectors