Matrix mettaloproteinase-3 as a potential biomarker for human metastatic osteosarcoma or Elevated expression of MMP-3 in human osteosarcoma and associated with tumor metastasis
Abstract:
Matrix metalloproteinase-3 (MMP-3, is one of several matrix metalloproteinases (MMPs) family that has been observed in several malignant tumors, including breast, colon, cervical and lung cancers, where its expression correlates with the invasion and metastasis of these tumors. However, the roles of MMP-3 in osteosarcoma are totally unknown. In this study, we examined the expression of MMP-3 in 15 primary and metastatic osteosarcomas with case-matched adjacent normal tissues by immunohistochemistry and quantitative RT-PCR. MMP-3 expression were expressed in 86.6 % (13/15) of the osteosarcoma tissues and the expression levels of MMP-3 were significantly higher in metastatic tumors than in primary osteosarcoma tumor tissues. Further, we investigated the expression of MMP-3 in osteoblast and osteosarcoma cells and found that MMP-3 was highly expressed in osteosarcoma cells compared to osteoblast cells. Knockdown of MMP-3 by siRNA in osteosarcoma cells significantly inhibited their migration and invasion properties. These findings suggest that MMP-3 expression is deregulated in osteosarcoma tumors, potentially contributing to metastasis and might be a promising marker for the prognosis and therapy of metastatic osteosarcoma.
Key words: Osteosarcoma, MMP-3, metastasis, EMT and invasion
“Once a dentist stopped me from asking a Nazi officer about my parents and I was really mad at him” This sentence refers to felix’s character because during a war where Jews are being killed and you are a Jew if you go around asking Nazis if they have seen your parents chances are you are going to get yourself murdered. FELIX'S NAIVETE “Once a dentist stopped me from asking a Nazi officer about my parents and I was really mad at Him” is an example of Felix’s naivete. Because Felix thinks just because he told the nazi officer a story he thinks the nazi cares about him even though the nazi has probably killed lots of Jews.
Osteosarcoma(OS) is a primary malignant tumor of bone which is characterized by the formation of osteoid tissue. Although it is the most common malignancy of long bones after multiple myeloma [2], it is a relatively rarer entity in the craniofacial region. About 6% of Oss arise in the jaws .The estimated incidence of the new cases of Jaw OS (JOS) per year is 0 .07 in 100,000. (1) The etiology of OS is unknown, but some risk factors such as a previous history of ionizing radiation, alkylating agent, retinoblastoma and benign bone lesions such as paget disease and fibro osseous dysplasia have been associated with the development of head and neck OS.(2-4) JOS occur with a peak in the third through fifth decades of life. The mean age is
This cancer is a bone cancer that attack the bones, especially large bones. This cancer is named osteogenic sarcoma(Osteosarcoma) for medical term (“Osteosarcoma”, n.d.). Osteosarcoma mostly affects people under 25 years old, and it can affect old people but it is really rare(“Osteosarcoma: An Introduction.”, 2012). When this cancer attacks, it grows bones, and any type of bone (“Bone cancer”,2013). This cancer is an ancient disease that we started to recognize in 1805. Most of the symptoms are on the bones (“Bone cancer”,2013). It also is not contagious so its not passed from person to person like other diseases.
Cancer is very powerful because cancer often travels to other parts of the body, and forms new tumors there. With osteosarcoma, if the cancer doesn’t spread, the survival rate is about 75%. If it spreads, the chances of survival are around 30%. Treatments can help in some cases, but other cases are fatal. Cancer is very powerful, and can kill humans because no cure has been found
Multiple myeloma is a haematological malignancy characterised by the suppression of osteoblastogenesis and the inability to form bone8. The inhibition of the Runt-related transcription factor 2 (Runx2) which is a key transcription factor responsible for osteoblast differentiation is largely implicated in multiple myeloma4. Runx2 stimulates the generation of the bone formation markers alkaline phosphatase (ALP), osteocalcin (OCN) and collagen during early osteoblast differentiation9,10. Transgenic mice without the Runx2 gene exhibit a lack of osteoblast formation causing the arrest of both endochondral and intramembranous ossification11. The upregulation of bone resorption and a decrease in bone mass is a common feature of multiple myeloma, presenting clinically as lytic bone lesions12.
during adolescent growth, which could be why 80% of all osteosarcoma childhood cases occur in the lower long bones and at the time of puberty (Mirabello & Savage 5). The chances of a cell dividing uncontrollably are increased at a time of rapid cell growth when there is many more cells dividing that could go suddenly go uncontrolled, and therefore the answer of how osteosarcoma is ignited could lie in puberty. On the contrary, the answer may be found in a small gene that prevents osteosarcoma patients from responding well to the chemotherapy treatment.
My last goal is to show my findings in a good and understandable fashion through this paper to help my readers understand more about Osteosarcoma. Audience Since osteosarcoma is a lesser known type of cancer, I want to tell about what it is, some symptoms of it, and why metastasized cancer is bad. By making my peers my specific audience, I will be able to help them better understand what osteosarcoma is and how it forms.
Rare and currently incurable multiple myeloma accounts for roughly 1% of overall cancers and 13% of hematological cancers (Palumbo & Anderson, 2011). This form of cancer involves the infiltration of malignant plasma cells into the bone marrow. The median age of diagnosed individuals equals roughly 70 years of age (2011). Multiple myeloma progression results in the deactivation of bone synthesizing osteoblasts and the activation of bone disassembling osteoclasts that cause osteolytic lesions that present as bone pain, fractures, and hypercalcemia (2011). In healthy human bone marrow, plasma cells usually comprise <5.5mg/mL showed a median survival of 44 months (2015). With current treatment
In this paper, Osteosarcoma will be taken apart by details. The symptoms, treatment, signs, and other details will be discussed and explained. In a normal bone for most people there are two types of cells; osteoblasts and osteoclasts. Osteoblasts are what build up our bones by forming the bone matrix and therefore gives us the strength in our bones. Osteoclasts on the other hand break down the bone matrix so that we don’t get too much of it and that helps the bones to keep its proper shape. In Osteosarcoma the osteoblasts are what help make up the cancer in the bones. The bones do not have as strong of a bone matrix. This type of cancer is most commonly found in kids and young adults. There are three forms of treatment but not
To illustrate the danger of canine osteosarcoma, imagine a ten-year-old Saint Bernard that has symptoms of bone cancer. The dog has extreme bone pain and loss of appetite. The dog’s owner and veterinarian decide to give the Saint Bernard pain medication. A few months later, the dog cannot walk and dies. Unfortunately, osteosarcoma is the most common type of bone cancer in larger dogs and there is no cure. The most effective way to treat canine osteosarcoma is with amputation and chemotherapy because it manages bone pain, improves the survival time, and increases the survival rate.
The likely site of PCa metastasis is the metabolically active hematopoietic bone marrow. Tombal and Lecouvet (2012) report that bone metastases, specifically into the hematopoietic red marrow, represents the main and primary site of metastasis for 80% of PCa’s. The abundant vasculature within bone marrow provides malignant cells with ample oxygen and nutrients that are needed for successful establishment of a metastic lesion. Additionally, the nearby pelvic lymph nodes provide PCa with an efficient route of metastasis into the pelvis, hips, and lumbar vertebrates. Tumour cells achieve absorption into bone morrow through a process that is directed by tumour cells, but effected by osteoclasts (Koutsilieris et al., 2006). Tumour cells
The uncontrollable spread of cancer is the principal event which leads to the death in individuals with cancer and it is the greatest barrier of developing cures for cancer. Metastasis is the progressive spread of malignant cancer cells from the primary tumour to secondary organ in distant sites and this potential is dependent on the specific microenvironment which support them to complete each step of the metastatic process (Poste & Fidler 1980). To understand the molecular basis of metastasis, investigators have now separated the complex and highly selective metastasis process into series of steps to try and solve the problems cause by
Cancer is listed as the second most common cause of death in western countries; particularly, in adults. Though it has a long antiquity, its prevalence and incidence today is pervasive and the war on cancer has not been promising. Malignant neoplasia is characterized by uncontrolled growth and the ability to metastasize or spread from the original site. Cancer results from mutations that promote cell proliferation and inhibit cell adhesion (metastasis). According to the National Cancer Institute (2016), “Cancer can also spread regionally,
In My opinion was caused by assassination/genetic impairments. To back up that statement there is information that proves he was assassinated due to his family tree and genetic impairments.
Osteosarcoma is an ancient disease that still has some mystery behind it. Osteosarcoma is a type of cancer that starts in the bones. It is also the most common type of bone cancer, and makes up 65% of all bone cancer. However, it is a very rare cancer and has fewer than 20,000 cases per year in The USA. The cells that form an osteosarcoma make bone matrix, similar to osteoblasts. However, the bone matrix of an osteosarcoma is not as strong as a bone matrix from an osteoblast, and therefore is not as strong as normal bones. The most common age group affected by osteosarcoma is children and young adults. However, osteosarcoma can occur at any age. Osteosarcoma is most commonly found in areas of the bone that grow quickly, which is why children are more likely to get this type of cancer. The most common place to find osteosarcoma is the end of long bones, especially in the knee, distal femur, and proximal tibia. The proximal humerus is typically the most common site. The treatments of